caa a22 4500 477120431 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00229473 doi (NATIONALLICENCE)springer-10.1007/BF00229473 Vitamin A inhibits lipoperoxidation ascorbate-Fe++ dependent of rat kidney microsomes and mitochondria [Elektronische Daten] [V. Piergiacomi, A. Palacios, A. Catalá] In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe++ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect. Kluwer Academic Publishers, 1996 lipoperoxidation nationallicence microsomes nationallicence mitochondria nationallicence rat kidney nationallicence Piergiacomi V. Assistant of the Comisión de Investigaciones Científicas y Técnicas de la Prov. de Buenos Aires (CIC), Argentina aut Palacios A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata CC 296, 1900 La Plata, Argentina aut Catalá A. Member of Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/2(1996-12-01), 121-125 0300-8177 165:2<121 1996 165 11010 https://doi.org/10.1007/BF00229473 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229473 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Piergiacomi V. Assistant of the Comisión de Investigaciones Científicas y Técnicas de la Prov. de Buenos Aires (CIC), Argentina aut NATIONALLICENCE 700 1- Palacios A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata CC 296, 1900 La Plata, Argentina aut NATIONALLICENCE 700 1- Catalá A. Member of Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/2(1996-12-01), 121-125 0300-8177 165:2<121 1996 165 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer