caa a22 4500 477120474 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00229471 doi (NATIONALLICENCE)springer-10.1007/BF00229471 Glycosylation is critical for natriuretic peptide receptor-B function [Elektronische Daten] [Randy Fenrick, Normand McNicoll, André De Léan] Co-transfection of a truncated natriuretic peptide receptor-B (NPR-B) with the full length receptor results in a decrease of 60-80% in wild-type receptor activity. This reduction correlates with a loss of glycosylation of the full length NPR-B. This effect is dose-dependent, and occurs with no change in the glycosylation of the truncated receptor. Co-transfection of the full length NPR-B with other receptors yields similar results. These data suggest that glycosylation may be crucial for NPR-B function. Cross-linking studies further demonstrate that only fully glycosylated NPR-B receptors are able to bind ligand. Our data therefore argue that carbohydrate modification may be critical for NPR-B receptor ligand binding. Kluwer Academic Publishers, 1996 C-type natriuretic peptide nationallicence natriuretic peptide receptor-B nationallicence guanylyl cyclase nationallicence N-linked glycosylation nationallicence as : amino acids nationallicence ANF : atrial natriuretic factor nationallicence ANOVA : analysis of variance nationallicence BS3 : bis(sulfosuccinimidyl) suberate nationallicence BSA : bovine serum albumin nationallicence CNP : C-type natriuretic peptide nationallicence DEAE : dextran-diethylaminoethyl-dextran nationallicence DMEM : Dulbecco's modified Eagle medium nationallicence DMSO : dimethyl sulfoxide nationallicence dNTP : deoxynucleotide triphosphate nationallicence EDTA : ethylenediamine tetraacetic acid nationallicence IBMX : 3-isobutyl-l-methyl-=xanthine nationallicence min : minutes nationallicence N-linked : asparagine-linked nationallicence NPR : natriuretic peptide receptor nationallicence nt : nucleotide nationallicence PCR : polymerase chain reaction nationallicence RIA : radioimmunoassay nationallicence RP-HPLC : reverse phase-high performance liquid chromatography nationallicence RP-HPLC : reverse phase-high performance liquid chromatography nationallicence SDS : sodium dodecyl sulfate nationallicence UV : ultraviolet nationallicence Fenrick Randy Department of Biochemistry, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut McNicoll Normand Department of Pharmacology, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut De Léan André Department of Pharmacology, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/2(1996-12-01), 103-109 0300-8177 165:2<103 1996 165 11010 https://doi.org/10.1007/BF00229471 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229471 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fenrick Randy Department of Biochemistry, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- McNicoll Normand Department of Pharmacology, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- De Léan André Department of Pharmacology, University of Montreal, H3C3J7, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/2(1996-12-01), 103-109 0300-8177 165:2<103 1996 165 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer