caa a22 4500 477120512 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408669 doi (NATIONALLICENCE)springer-10.1007/BF00408669 Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction [Elektronische Daten] [Angel Zarain-Herzberg, Nasir Afzal, Vijayan Elimban, Naranjan Dhalla] Myocardial infarction in rats induced by occluding the left coronary artery for 4, 8 and 16 weeks has been shown to result in congestive heart failure (CHF) characterized by hypertrophy of the viable ventricular myocardial tissue. We have previously demonstrated a decreased calcium transport activity in the sarcoplasmic reticulum (SR) of post-myocardial infarction failing rat hearts. In this study we have measured the steady state levels of the cardiac SR Ca2+-pump ATPase (SERCA2) mRNA using Northern blot and slot blot analyses. The relative amounts of SERCA2 mRNA were decreased with respect to GAPDH mRNA and 28 S rRNA in experimental failing hearts at 4 and 8 weeks post myocardial infarction by about 20% whereas those at 16 weeks declined by about 35% of control values. The results obtained by Western blot analysis, revealed that the immunodetectable levels of SERCA2 protein in 8 and 16 weeks postinfarcted animals were decreased by about 20% and 30%, respectively. The left ventricular SR Ca2+-pump ATPase specific activity was depressed in the SR preparations of failing hearts as early as 4 weeks post myocardial infarction and declined by about 65% at 16 weeks compared to control. These results indicate that the depressed SR Ca2+-pump ATPase activity in CHF may partly be due to decreased steady state amounts of SERCA2 mRNA and SERCA2 protein in the failing myocardium. Kluwer Academic Publishers, 1996 Ca2+-pump ATPase nationallicence cardiac SERCA2 nationallicence myocardial infarction nationallicence congestive heart failure nationallicence cardiac SR nationallicence Zarain-Herzberg Angel Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Afzal Nasir Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Elimban Vijayan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Dhalla Naranjan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 285-290 0300-8177 163-164:1<285 1996 163-164 11010 https://doi.org/10.1007/BF00408669 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408669 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zarain-Herzberg Angel Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Afzal Nasir Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Elimban Vijayan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Dhalla Naranjan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 285-290 0300-8177 163-164:1<285 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer