caa a22 4500 477120520 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408660 doi (NATIONALLICENCE)springer-10.1007/BF00408660 Hypertrophic responsiveness to β2-adrenoceptor stimulation on adult ventricular cardiomyocytes [Elektronische Daten] [Xi Zhou, Klaus-Dieter Schlüter, Hans Piper] The aim of the present study was to characterize the receptor subtype and the second messenger involved in the newly discovered hypertrophic effect of β-adrenoceptor stimulation in cultures of adult ventricular cardiomyocytes. Cardiomyocytes isolated from adult rats and cultured for 6 days in presence of 20% fetal calf serum (FCS) were used as experimental model. Hypertrophic responsiveness of cardiomyocytes was characterized by rate of protein synthesis, increase in protein mass, and increase in RNA content. The hypertrophic effect of the non-specific β-adrenoceptor agonist isoprenaline was abolished in presence of a specific β1-adrenoceptor antagonist (ICI 118,551), could be mimicked by use of a β2-adrenoceptor agonist (procaterol) or direct stimulation of adenylate cyclase (forskolin) or addition of a cell-permeable analogue of cAMP (dibuytyrylcyclo-AMP). In presence of Rp-cAMPS, an inhibitor of protein kinase A, the hypertrophic effect of isoprenaline was abolished. The results indicate that the hypertrophic effect of β-adrenoceptor stimulation is due to stimulation of β2-adrenoceptors and activation of adenylate cyclase and protein kinase A. Kluwer Academic Publishers, 1996 adenylate cyclase nationallicence protein synthesis nationallicence transforming growth factor-β nationallicence protein kinase A nationallicence Zhou Xi Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut Schlüter Klaus-Dieter Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut Piper Hans Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 211-216 0300-8177 163-164:1<211 1996 163-164 11010 https://doi.org/10.1007/BF00408660 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408660 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Xi Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut NATIONALLICENCE 700 1- Schlüter Klaus-Dieter Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut NATIONALLICENCE 700 1- Piper Hans Physiologisches Institut, Justus-Liebig-Universität, Aulweg 129, D-35392, Giessen, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 211-216 0300-8177 163-164:1<211 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer