caa a22 4500 477120547 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408657 doi (NATIONALLICENCE)springer-10.1007/BF00408657 Effects of anti-peptide antibodies against the second extracellular loop of human M2 muscarinic acetylcholine receptors on transmembrane potentials and currents in guinea pig ventricular myocytes [Elektronische Daten] [Rongrui Zhao, Wenze Wang, Bowei Wu, Johan Hoebeke, Åke Hjalmarson, Michael Fu] The effects of anti-peptide antibodies against the second extracellular loop of human M2 muscarinic receptor on transmembrane potentials and currents in guinea pig single ventricular cells were analyzed using whole-cell patch clamp technique. These effects were compared with those of the muscarinic receptor agonists carbachol and acetylcholine. The antibodies shortened the action potential duration in a dose-dependent manner. By using a ramp or step rectangular pulse protocol, it was found that the antibodies increased the outward K+ current and decreased the inward basal I Ca significantly. The reversal potential of both carbachol-and antibody-induced extra currents were close to −80 mV, being in proximity to the calculated Ek of −90 mV. A β-adrenergic receptor agonist, isoprenaline, prolonged the action potential and increased the overshoot which could be inhibited by both antibody and carbachol. Isoprenaline increased inward Ica and outward Ik simultaneously. Both antibody and carbachol could significantly reduce the isoprenaline-stimulated ICa but not the isoprenaline-stimulated Ik. The antibody- or carbachol-induced outward K+ current and the depressant effects of antibody and carbachol on isoprenaline-stimulated Ica were partially antagonized by atropine. These results suggest that the anti-M2 muscarinic receptor antibodies display a stimulatory activity similar to muscarinic receptor agonist on the receptor-mediated electrophysiological events. Kluwer Academic Publishers, 1996 antibody nationallicence outward K+ current nationallicence inward Ca2− current nationallicence patch clamp nationallicence Zhao Rongrui Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut Wang Wenze Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut Wu Bowei Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut Hoebeke Johan Laboratoire d'Enzymologie et de Chimie des Proteines, URA 1334 CNRS, F 37032, Tours, France aut Hjalmarson Åke Wallenberg Laboratory, Sahlgren's Hospital, University of Goteborg, 413 45, Goteborg, Sweden aut Fu Michael Wallenberg Laboratory, Sahlgren's Hospital, University of Goteborg, 413 45, Goteborg, Sweden aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 185-193 0300-8177 163-164:1<185 1996 163-164 11010 https://doi.org/10.1007/BF00408657 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408657 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Rongrui Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Wang Wenze Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Wu Bowei Department of Physiology, Shanxi Medical College, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Hoebeke Johan Laboratoire d'Enzymologie et de Chimie des Proteines, URA 1334 CNRS, F 37032, Tours, France aut NATIONALLICENCE 700 1- Hjalmarson Åke Wallenberg Laboratory, Sahlgren's Hospital, University of Goteborg, 413 45, Goteborg, Sweden aut NATIONALLICENCE 700 1- Fu Michael Wallenberg Laboratory, Sahlgren's Hospital, University of Goteborg, 413 45, Goteborg, Sweden aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 185-193 0300-8177 163-164:1<185 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer