caa a22 4500 477120555 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408639 doi (NATIONALLICENCE)springer-10.1007/BF00408639 Localization of α1,2,3-subunit isoforms of Na,K-ATPase in cultured neonatal and adult rat myocardium: The immunofluorescence and immunocytochemical study [Elektronische Daten] [Jan Slezak, Wolfgang Schulze, Zuzana Stefankova, Ludmila Okruhlicova, Ludovit Danihel, Gerd Wallukat] By indirect immunofluorescence and preembedding peroxidase-diaminobenzidine technique the localization of polyclonal and monoclonal antibodies against α1, α2 and α3 isoforms of the Na,K-ATPase were studied in rat myocardium. The α1-subunit was identified predominantly on sarcolemma of cultured myocytes, neonatal, as well as adult cardiocytes. The α2 signal was localized around nuclei of cultured cardiocytes, very weak signals were seen in neonatal and more intense signal, were dispersed throughout the adult myocytes. The α3-subunit immunoreactivity was weak and localized in cell processes connecting individual cultured cells, on sarcolemma and intercalated discs of neonatal cells and very weak in adult working myocytes. Cytochemically demonstrated ouabain resistant Na,K-ATPase localized in junctional sarcoplasmic reticulum may represent α1 isoenzyme which is directly involved in modulation of action potential fluxes. Kluwer Academic Publishers, 1996 Na,K-ATPase nationallicence localization nationallicence immunofluorescence nationallicence immunoelectron microscopy nationallicence Slezak Jan The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Schulze Wolfgang Max-Delbruck -Center for Molecular Medicine, Cardiology, Berlin, Germany aut Stefankova Zuzana The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Okruhlicova Ludmila The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Danihel Ludovit Institute of Pathology Medical Faculty, Comenius University, Bratislava, Slovak Republic aut Wallukat Gerd Max-Delbruck -Center for Molecular Medicine, Cardiology, Berlin, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 39-45 0300-8177 163-164:1<39 1996 163-164 11010 https://doi.org/10.1007/BF00408639 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408639 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Slezak Jan The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Schulze Wolfgang Max-Delbruck -Center for Molecular Medicine, Cardiology, Berlin, Germany aut NATIONALLICENCE 700 1- Stefankova Zuzana The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Okruhlicova Ludmila The Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Danihel Ludovit Institute of Pathology Medical Faculty, Comenius University, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Wallukat Gerd Max-Delbruck -Center for Molecular Medicine, Cardiology, Berlin, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 39-45 0300-8177 163-164:1<39 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer