caa a22 4500 477120563 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408645 doi (NATIONALLICENCE)springer-10.1007/BF00408645 In-vivo phosphorylation of the cardiac L-type calcium channel beta-subunit in response to catecholamines [Elektronische Daten] [Hannelore Haase, Sabine Bartel, Peter Karczewski, Ingo Morano, Ernst-Georg Krause] In canine myocardium, the β-subunit of the L-type Ca2+ channel is phosphorylated by cAMP dependent protein kinase in vitro as well as in vivo (Haase et al. FEBS Lett 335: 217-222, 1993). We have assessed the identity of the β-subunit as well as its in vivo phosphorylation in representative experimental groups of catecholamine-challenged canine hearts. Adrenergic stimulation by high doses of both noradrenaline and isoprenaline induced rapid (within 20 sec) and nearly complete phosphorylation of the Ca2+ channel β-subunit. Phosphorylation in vivo was about 4-fold higher as compared to untreated controls. When related to catecholamine-depleted (reserpine-treated) hearts noradrenaline and isoprenaline increased the in vivo phosphorylation of the β-subunit even 8-fold. This phosphorylation correlated positively with tissue levels of cAMP, endogenous particulated cAMP-dependent protein kinase (PKA) and the rate of contractile force development dP/dtmax. The results imply the involvement of a PKA-mediated phosphorylation of the Ca2+ channel β-subunit in the adrenergic stimulation of intact canine myocardium. Kluwer Academic Publishers, 1996 Ca2+ channels nationallicence CAMP-dependent phosphorylation nationallicence cardiac contractile force nationallicence protein kinase A nationallicence Haase Hannelore Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut Bartel Sabine Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut Karczewski Peter Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut Morano Ingo Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut Krause Ernst-Georg Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 99-106 0300-8177 163-164:1<99 1996 163-164 11010 https://doi.org/10.1007/BF00408645 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408645 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haase Hannelore Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut NATIONALLICENCE 700 1- Bartel Sabine Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut NATIONALLICENCE 700 1- Karczewski Peter Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut NATIONALLICENCE 700 1- Morano Ingo Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut NATIONALLICENCE 700 1- Krause Ernst-Georg Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 99-106 0300-8177 163-164:1<99 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer