caa a22 4500 47712058X CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408665 doi (NATIONALLICENCE)springer-10.1007/BF00408665 Heterotopic heart transplantation alters high-energy phosphate metabolism irrespective of cardiac allograft rejection [Elektronische Daten] [J. van Dobbenburgh, Carina Kasbergen, Piet Slootweg, Tom Ruigrok, Cees van Echteld] This study was undertaken to validate the potential of 31P magnetic resonance spectroscopy (MRS) as a noninvasive alternative for transvenous endomyocardial biopsy in detecting cardiac allograft rejection. Donor hearts from either Lewis rats (L) or Brown-Norway rats (BN) were transplanted into the neck of L rats resulting in a non-rejecting group L-L and a rejecting group L-BN. L-L and L-BN rats were serially studied by means of 31P MRS from postoperatine day 1-8. In addition, rejection was confirmed by histology. A similar, marked decrease in phosphocreative/β-adenosinetriphosphate (PCr/ATP) ratio from day 1-3 was observed in both L-L and L-BN hearts. This ratio levelled off on postoperative day 3 and remained depressed on subsequent postoperative days in both groups, although histology showed an increase in the severity of rejection in L-BN. However, the PCr signal/noise ratio in L-BN started to decrease after day 4, coinciding with the histologic evidence of severe rejection (score IV), whereas in L-L hearts (score 0) this ratio remained unaltered until day 8. Since high-energy phosphate metabolism is affected by the unloaded status of the heterotopically transplanted heart, irrespective of rejection, the PCr/ATP ratio appears not to be a specific marker for the detection of acute rejection in this model. In contrast, the PCr S/N ratio appears to be a specific and sensitive marker of acute rejection, but only in a late, severe stage. Kluwer Academic Publishers, 1996 high-energy phosphate metabolism nationallicence magnetic resonance spectroscopy nationallicence heart transplantation nationallicence allograft rejection nationallicence van Dobbenburgh J. Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut Kasbergen Carina Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut Slootweg Piet Department Of Pathology, University Hospital, Utrecht, The Netherlands aut Ruigrok Tom Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut van Echteld Cees Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 247-252 0300-8177 163-164:1<247 1996 163-164 11010 https://doi.org/10.1007/BF00408665 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408665 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Dobbenburgh J. Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Kasbergen Carina Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Slootweg Piet Department Of Pathology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Ruigrok Tom Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Echteld Cees Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 247-252 0300-8177 163-164:1<247 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer