caa a22 4500 47712061X CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408653 doi (NATIONALLICENCE)springer-10.1007/BF00408653 Localization of α1-adrenoceptors in rat and human hearts by immunocytochemistry [Elektronische Daten] [Wolfgang Schulze, Michael Fu] The localization of the ai adrenoceptors (α1-AR) in the heart tissues from rat and human and in the cultured heart cells from neonatal rats was studied by indirect immunofluorescence and postembedding electronmicroscopical immuno-gold technique. With antipeptide antibodies directed against the second extracellular loop of the human α1-AR (AS sequence 192-218), this receptor was found to be localized along the sarcolemma in both human and rat hearts. Similar localization sites were detected in cultivated rat neonatal cardiomyocytes. Beside the localization in cardiomyocytes, α1-AR were identified in endothelial cells of capillaries and smooth muscle cells of coronary vessels, in neuronal endings, in mast cells of cultivated heart cells but not, or in less amount in fibroblasts. Interestingly, in the right atrium of rat heart the localization of α1-AR was found to be near or on atrial natriuretic factor (ANF) granules, providing the basis for the α-adrenergic influence on ANF release. The immunocytochemical studies further confirm and complete the findings known by using autoradiographic binding studies with specific ligands. Kluwer Academic Publishers, 1996 immunocytochemistry nationallicence antipeptide antibodies nationallicence α1-adrenoceptors nationallicence heart cells nationallicence Schulze Wolfgang Max-Delbrück-Centre for Molecular Medicine, Cardiology, Berlin, Germany aut Fu Michael Wallenberg Laboratory, Sahlgren's Hospital, University Goteborg, Goteborg, Sweden aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 159-165 0300-8177 163-164:1<159 1996 163-164 11010 https://doi.org/10.1007/BF00408653 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408653 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schulze Wolfgang Max-Delbrück-Centre for Molecular Medicine, Cardiology, Berlin, Germany aut NATIONALLICENCE 700 1- Fu Michael Wallenberg Laboratory, Sahlgren's Hospital, University Goteborg, Goteborg, Sweden aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 159-165 0300-8177 163-164:1<159 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer