caa a22 4500 477120636 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408649 doi (NATIONALLICENCE)springer-10.1007/BF00408649 Cardiac pump function of the isolated rat heart at two modes of energy deprivation and effect of adrenergic stimulation [Elektronische Daten] [Valeri Kapelko, Vladimir Lakomkin, Olga Korchazhkina, Oleg Pisarenko] The contractile function of the isolated rat heart and high energy phosphate content were evaluated under conditions of depressed energy supply caused by disturbances either in mitochondrial ATP production or ATP-phosphocreatine transformation. Amytal (0.3 mM), an inhibitor of mitochondrial respiration, or iodoacetamide (IAA, 0.1 mM) reducing in this dose creatine kinase activity to 19% of the initial level, were used, respectively. Myocardial ATP content remained unaffected in both groups and PCr content decreased to 37% only in amytal-treated group. Very similar alterations in cardiac pump function during volume load were observed in both treated groups; maximal cardiac output was significantly less by 30%, cardiac pressure-volume work by 38-40%, left ventricular (LV) systolic pressure by 24-29%, and LV +dP/dt by 36-39%. In contrast, the extent of decreased LV distensibility was different, a curve relating LV filling volume and end-diastolic pressure was shifted up and to the left much more prominently after IAA treatment. Heart rate was decreased by 24% only in amytal-treated group. Results indicate that a decreased myocardial distensibility is a dominating feature in the acute cardiac pump failure caused by an inhibition of myocardial creatine kinase. Isoproterenol (0.1 μM) substantially increased heart rate and pressure-rate product in IAA-treated hearts but failed to increase cardiac work probably due to its inability to improve myocardial distensibility. Kluwer Academic Publishers, 1996 isolated heart nationallicence pump function nationallicence distensibility nationallicence energy supply nationallicence isoproterenol nationallicence high energy phosphates nationallicence Kapelko Valeri Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut Lakomkin Vladimir Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut Korchazhkina Olga Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut Pisarenko Oleg Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 131-136 0300-8177 163-164:1<131 1996 163-164 11010 https://doi.org/10.1007/BF00408649 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408649 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kapelko Valeri Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Lakomkin Vladimir Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Korchazhkina Olga Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Pisarenko Oleg Cardiology Research Center, 15A Cherepkovskaya Street, 121552, Moscow, Russia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 131-136 0300-8177 163-164:1<131 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer