caa a22 4500 477120652 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408674 doi (NATIONALLICENCE)springer-10.1007/BF00408674 Circulating antibodies against neonatal cardiac muscarinic acetylcholine receptor in patients with Sjögren's syndrome [Elektronische Daten] [Enri Borda, Juan Camusso, Claudia Perez Leiros, Sandra Bacman, Osvaldo Hubscher, Roberto Arana, Leonor Sterin-Borda] Isolated congenital heart block may be associated with Primary Sjogren's Syndrome. In this work we demonstrated that IgG present in the sera ofpatients with Primary Sjogren's Syndrome (PSS) could bind and activate muscarinic acetylcholine receptors of rat neonatal atria. These antibodies were able to inhibit in a irreversible manner the binding of 3H-QNB to muscarinic cholinergic receptors of purified rat atria membranes. Moreover, IgG from PSS individuals could modify biological effects mediated by muscarinic cholinoceptors activation, i.e. decrease contractility and cAMP and increase phosphoinositide turnover and cGMP. Atropine blocked all of these effects and carbachol mimicked them; confirming muscarinic cholinergic receptors-mediated PSS IgG action. Neither binding nor biological effect were obtained using adult instead of neonatal rat atria. IgG from sera of normal women were not effective in the studied system. The prevalence of cholinergic antibody was 100% in PSS and was independent of Ro/SS-A and La/SS-B antibodies. It could be concluded that antibody against muscarinic cholinergic receptors may be another serum factor to be considered in the pathophysiology of the development of congenital heart block. Kluwer Academic Publishers, 1996 muscarinic cholinergic antibodies nationallicence binding assay nationallicence atria contractility nationallicence cAMP and cGMP production nationallicence phosphoinositide hydrolysis nationallicence Borda Enri CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Camusso Juan CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Perez Leiros Claudia CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Bacman Sandra CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Hubscher Osvaldo CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Arana Roberto CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Sterin-Borda Leonor CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 335-341 0300-8177 163-164:1<335 1996 163-164 11010 https://doi.org/10.1007/BF00408674 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408674 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borda Enri CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Camusso Juan CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Perez Leiros Claudia CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Bacman Sandra CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Hubscher Osvaldo CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Arana Roberto CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Sterin-Borda Leonor CEFYBO-CONICET School of Dentistry, University of Buenos Aires and CEMIC, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 335-341 0300-8177 163-164:1<335 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer