caa a22 4500 477120717 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408643 doi (NATIONALLICENCE)springer-10.1007/BF00408643 Role of bradykinin in the antihypertr. ophic effects of enalapril in the newborn pig heart [Elektronische Daten] [Cathy Beinlich, Christine Rissinger, Kristen Vitkauskas, Howard Morgan] Rapid growth of the left ventricle of the newborn pig heart can be restrained by treating piglets with the angiotensin converting enzyme inhibitor, enalapril maleate. This reduced rate of growth is reflected in vitro by reduced rates of ribosome formation and protein synthesis, and may be due to decreased availability of angiotensin II (All), a potentially hypertrophic agent; decreased numbers of All receptors; increased availability of bradykinin, a potentially antihypertrophic agent; or reduced hemodynamic load on the left ventricle. Because enalapril decreases degradation of bradykinin, the role of bradykinin as an inhibitor of cardiac growth in the newborn heart was investigated. Addition of 1 × 10−5 M bradykinin and 1 × 10−6 Menalapril to the perfusate of isolated hearts from 2 day old piglets did not significantly alter heart rate, contents of ATP or creatine phosphate or rates of ribosome formation or protein synthesis during 1 h of perfusion. Similarly, exposure of myocytes isolated from the left ventricular free wall of piglets to 5 × 10−6 M bradykinin for 72 h did not alter the rate of [3H]-phenylalanine incorporation into total protein. The reduced rate of left ventricular growth in vivo caused by enalapril administration was not reversed by simultaneous treatment with the specific bradykinin receptor antagonist, HOE 140. HOE 140 alone did not alter ventricular growth as compared to hearts from untreated piglets. In summary, these results demonstrate that the reduced rate of left ventricular growth in vivo and the reduced rate of ribosome formation and protein synthesis in the left ventricle in vitro after enalapril treatment of piglets is not the result of an inhibitory effect of bradykinin on cardiac growth. Kluwer Academic Publishers, 1996 bradykinin nationallicence HOE 140 nationallicence enalapril maleate nationallicence hypertrophy nationallicence angiotensin 11 nationallicence newborn heart nationallicence Beinlich Cathy Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut Rissinger Christine Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut Vitkauskas Kristen Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut Morgan Howard Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 77-83 0300-8177 163-164:1<77 1996 163-164 11010 https://doi.org/10.1007/BF00408643 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408643 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beinlich Cathy Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut NATIONALLICENCE 700 1- Rissinger Christine Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut NATIONALLICENCE 700 1- Vitkauskas Kristen Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut NATIONALLICENCE 700 1- Morgan Howard Weis Center for Research, Geisinger Clinic, 18722, Danville, PA, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 77-83 0300-8177 163-164:1<77 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer