caa a22 4500 477120733 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408641 doi (NATIONALLICENCE)springer-10.1007/BF00408641 Early postnatal changes in sarcoplasmic reticulum calcium transport function in spontaneously hypertensive rats [Elektronische Daten] [Nicholas Freestone, Jaipaul Singh, Ernst-Georg Krause, Roland Vetter] This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spo'sntaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal development at days 1, 3, 6, 12 and at day 40 to ascertain any difference in SR Ca2+ handling before the onset of hypertension. At day 1, the rate of homogenate oxalate-supported Ca2+ uptake was significantly higher in SHR than in WKY (0.25 ± 0.02 vs 0.12 ± 0.01 nmoles Ca2+/mg wet ventricular weight/min, respectively; p < 0.001). This interstrain difference disappeared with further developmental increase in SR Ca2+ transport. Western Blot analysis and a semiquantitative ELISA did not reveal any difference in the amount of immunoreactive PLB (per mg of total tissue protein) between strains at any of the ages studied. In addition, levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic (C) subunit of protein kinase A did not differ between SHR and WKY at days 1, 3, 6 and 12. At day 40, C subunit-catalyzed formation of 32P-PLB was reduced by 66% (p < 0.001) in SHR when compared to age-matched WKY In the early postnatal period between day 1 and 12 SR Ca2+-transport values were linearly related to the respective 32P-PLB levels of both SHR and WKY rats. The results indicate that cardiac SR of SHR can sequester Ca2+ at a much higher rate immediately after birth compared to WKY rats. The disappearance of this interstrain difference with further development suggests that some endogenous neuroendocrine or nutritional factor(s) from the hypertensive mother may exert an influence upon the developing heart in utero resulting in a transiently advanced maturation of the SR Ca2+ transport function in SHR pups at the time of birth. Kluwer Academic Publishers, 1996 heart nationallicence postnatal development nationallicence sarcoplasmic reticulum nationallicence phospholamban nationallicence calcium transport nationallicence spontaneously hypertensive rats nationallicence growth nationallicence Freestone Nicholas Department of Applied Biology, University of Central Lancashire, PRI 2HE, Preston, UK aut Singh Jaipaul Department of Applied Biology, University of Central Lancashire, PRI 2HE, Preston, UK aut Krause Ernst-Georg Max Delbrück Center for Molecular Medicine, Robert Rössle Straße 10, D-13122, Berlin-Buck, Germany aut Vetter Roland Max Delbrück Center for Molecular Medicine, Robert Rössle Straße 10, D-13122, Berlin-Buck, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 57-66 0300-8177 163-164:1<57 1996 163-164 11010 https://doi.org/10.1007/BF00408641 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408641 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Freestone Nicholas Department of Applied Biology, University of Central Lancashire, PRI 2HE, Preston, UK aut NATIONALLICENCE 700 1- Singh Jaipaul Department of Applied Biology, University of Central Lancashire, PRI 2HE, Preston, UK aut NATIONALLICENCE 700 1- Krause Ernst-Georg Max Delbrück Center for Molecular Medicine, Robert Rössle Straße 10, D-13122, Berlin-Buck, Germany aut NATIONALLICENCE 700 1- Vetter Roland Max Delbrück Center for Molecular Medicine, Robert Rössle Straße 10, D-13122, Berlin-Buck, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 57-66 0300-8177 163-164:1<57 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer