caa a22 4500 477120768 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408659 doi (NATIONALLICENCE)springer-10.1007/BF00408659 Catecholamines and cardiac growth [Elektronische Daten] [Mahesh Gupta, Madhu Gupta, Smilja Jakovcic, Radovan Zak] The present knowledge concerning the α- and β-adrenergic systems in the regulation of cardiac growth and gene expression in reviewed. To investigate the mechanism by which CAMP regulates the expression of cardiac genes we have used cultured myocytes derived from fetal rat hearts. We have shown previously that the addition of Br cAMP to the culture medium produced an increase in α-myosin heavy chain (α-MHC) mRNA level, in its rate of transcription as well as in the amount of V1 isomyosin. To characterize the promoter element(s) involved in cAMP responsive regulation of a-MHC expression we performed transient transfection analysis with a series of α-MHC gene promoter-CAT constructs. We have identified a 13 by E-box/M-CAT hybrid motif (EM element) which conferred a basal muscle specific and cAMP inducible expression of the α-MHC gene. Using mobility shift assay we have documented that one of the EM element binding protein is TEF-1. Moreover, by incubating cardiac nuclear extracts with the catalytic subunit of PK-A we have found that factor(s) binding to the EM element is a substrate for CAMP dependent phosphorylation. Kluwer Academic Publishers, 1996 adrenergic system nationallicence control of gene expression nationallicence α-myosin heavy chain nationallicence Gupta Mahesh Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut Gupta Madhu The Heart Institute for Children and Christ Hospital Medical Center, 60463, Oak Lawn, IL, USA aut Jakovcic Smilja Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut Zak Radovan Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 203-210 0300-8177 163-164:1<203 1996 163-164 11010 https://doi.org/10.1007/BF00408659 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408659 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gupta Mahesh Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut NATIONALLICENCE 700 1- Gupta Madhu The Heart Institute for Children and Christ Hospital Medical Center, 60463, Oak Lawn, IL, USA aut NATIONALLICENCE 700 1- Jakovcic Smilja Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut NATIONALLICENCE 700 1- Zak Radovan Departments of Medicine (Section of Cardiology), Pharmacological and Physiological Sciences and Organismal Biology and Anatomy, The University of Chicago, 60637, IL aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 203-210 0300-8177 163-164:1<203 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer