caa a22 4500 477120822 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408642 doi (NATIONALLICENCE)springer-10.1007/BF00408642 Cardiac phosphocreatine deficiency induced by GPA during postnatal development in rat [Elektronische Daten] [Václav Pelouch, František Kolář, Zaza Khuchua, Galina Elizarova, Marie Milerová, Bohuslav Ošt'ádall, Valdur Saks] The effect of chronic administration of β-guanidinopropionic acid (GPA) on the protein profiling, energy metabolism and right ventricular (RV) function was studied in the rat heart during the weaning and adolescence period. GPA was given in tap water (1-1.5%) using pair drink controls. The feeding of animals with GPA solution for a six week period resulted in elevation of heart to body weight ratio due to body growth retardation. GPA accumulated in the myocardium up to 67.37 ± 5.3 μmoles.g dry weight and the tissue content of total creatine, phosphocreatine and ATP was significantly decreased to 15%, 9% and 65% of control values respectively. Total activity of creatine kinase (CK) was not changed, but the proportion of mitochondrial (Mi) CK isoenzyme was decreased; the percentage of MB isoenzyme of CK was significantly higher. GPA treatment resulted in an elevation of the content of cardiac collagenous proteins and decrease of non-collagenous proteins in the heart; in parallel, a decrease of the collagen I to collagen III ratio was detected. The function of the RV was assessed using an isolated perfused heart with RV performing pressure-volume work. As compared to pair-drink controls, RV function was significantly impaired the GPA group: at any given right atrial filling pressure, the RV systolic pressure and the rate of pressure development were decreased by almost a factor of two. Elevation of the RV diastolic pressure with increasing pulmonary artery diastolic pressure was also significantly steeper in the GPA group which also showed decrease of cardiac output, especially at high outflow resistance. It may be assumed that chronic administration of GPA deeply influenced metabolic parameters, protein profiles and contractile function of the developing heart. On the other hand, concentrations of glucose, total lipids and triglycerides in blood plasma were not affected. All these data confirm the concept that the CK system is of central importance both for heart function and for the regulation of normal growth of cardiac myocytes. Kluwer Academic Publishers, 1996 beta-guanidinopropionic acid nationallicence photocreatine deficiency nationallicence immature heart nationallicence myocardial protein profiling nationallicence collagen types nationallicence perfused heart nationallicence contractile function nationallicence metabolites in blood plasma nationallicence Pelouch Václav Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut Kolář František Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut Khuchua Zaza Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut Elizarova Galina Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut Milerová Marie Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut Ošt'ádall Bohuslav Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut Saks Valdur Laboratory of Bioenergetics, Institute of Chemical and Biological Physics, Ravala 10, EE 0001, Tallin, Estonia aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 67-76 0300-8177 163-164:1<67 1996 163-164 11010 https://doi.org/10.1007/BF00408642 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408642 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pelouch Václav Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Kolář František Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Khuchua Zaza Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Elizarova Galina Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Milerová Marie Institute of Experimental Cardiology, Cardiology Research Center, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Ošt'ádall Bohuslav Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Saks Valdur Laboratory of Bioenergetics, Institute of Chemical and Biological Physics, Ravala 10, EE 0001, Tallin, Estonia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 67-76 0300-8177 163-164:1<67 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer