caa a22 4500 477120849 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408671 doi (NATIONALLICENCE)springer-10.1007/BF00408671 Alterations in inotropy, nitric oxide and cyclic GMP synthesis, protein phosphorylation and ADP-ribosylation in the endotoxin-treated rat myocardium and cardiomyocytes [Elektronische Daten] [Prakash Sulakhe, Lakshman Sandirasegarane, J. Davis, Xuan Vo, Willard Costain, Rahul Mainra] To evaluate the effects of the in vivo endotoxin treatment of the rat on (1) the contractile responses in the subsequently isolated papillary muscle to adrenergic and cholinergic agonists and (2) the biochemical parameters (cyclic GMP, nitric oxide synthesis, protein phosphorylation and ADP-ribosyslation) in the subsequently isolated cardiomyocytes. Following the in vivo endotoxin treatment (4 mg/kg i.p., 18 h), contractile responses to increasing amounts of isoprenaline or to increasing amounts of oxotremorine in the presence of a fixed amount of isoprenaline were determined in isolated papillary strips. Activities of nitric oxide synthase, guanylyl cyclase, as well as phosphorylation of phospholamban and troponin-inhibitory subunit, and pertussis toxin-catalyzed and endogenous ADP-ribosylations were determined in the intact cardiomyocytes and subcellular fractions. The increase in the force of contraction by isoprenaline was reduced, while its inhibition by oxotremorine was greater in the endotoxin-treated papillary strips. The activities of both nitric oxide synthase, primarily of the inducible form of the enzyme, and cytosolic guanylyl cyclase were higher while the phosphorylations of both phospholamban and troponin-inhibitory subunit were of lesser magnitude in the cardiomyocytes following the in vivo endotoxin treatment. Pertussis toxin-catalyzed ADP-ribosylation of the 41 kDa polypeptide, which is the alpha subunit of Gi, was also decreased. The results of the present study support the postulate that alterations in both the cyclic AMP and cyclic GMP signalling cascade contribute to the myocardial dysfunction caused by endotoxin and cytokines. Kluwer Academic Publishers, 1996 nitric oxide nationallicence endotoxin nationallicence cardiomyocytes nationallicence guanosine 3′, 5′-cyclic monophosphate nationallicence calcium nationallicence ADP-ribosylation nationallicence phosphorylation nationallicence Sulakhe Prakash Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Sandirasegarane Lakshman Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Davis J. Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Vo Xuan Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Costain Willard Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Mainra Rahul Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 305-318 0300-8177 163-164:1<305 1996 163-164 11010 https://doi.org/10.1007/BF00408671 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408671 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sulakhe Prakash Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 700 1- Sandirasegarane Lakshman Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 700 1- Davis J. Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 700 1- Vo Xuan Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 700 1- Costain Willard Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 700 1- Mainra Rahul Department of Physiology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 305-318 0300-8177 163-164:1<305 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer