caa a22 4500 47712092X CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00408656 doi (NATIONALLICENCE)springer-10.1007/BF00408656 Characterization of catecholamine uptake2 in isolated cardiac myocytes [Elektronische Daten] [O. Obst, H. Rose, H. Kammermeier] Extraneuronal catecholamine uptake was investigated in isolated quiescent rat myocardial cells. By administration of (3H-)(−)noradrenaline concentration of 22 nmol/l up to 1000 μmol/l the following data were obtained: (1) The KM of the uptake process amounted to 260 μmol/l, the Vmax to 4.24 nmol/(10 min × mg Protein) corresponding to 179 nmol/(min × gWWt)(WWT = Wet Weight). (2) The uptake was largely inhibited by the uptake2-inhibitors corticosterone (100 μmol/l), isoprenaline (IC so = 30.6 μmol/l), and O-methylisoprenaline (IC50 = 2.1 pmol/l), but not by the uptake1-inhibitors cocaine (100 μmol/l) and desipramine (10 μmol/l). (3) The ‘affinity'-values KM and IC50 closely agreed with those already known, but the Vmax-value was higher than those obtained in whole rat hearts by a factor of at least 1.79. This is caused presumably by the voltage dependence of the uptake mechanism and the resulting inhibition of uptake 2 during the periods of depolarisation in beating hearts of other studies. Kluwer Academic Publishers, 1996 catecholamine uptake2 nationallicence isolated cardiomyocytes nationallicence membrane potential nationallicence noradrenaline nationallicence Obst O. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut Rose H. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut Kammermeier H. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 181-183 0300-8177 163-164:1<181 1996 163-164 11010 https://doi.org/10.1007/BF00408656 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00408656 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Obst O. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Rose H. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Kammermeier H. Medical Faculty, RWTHAachen, Institute of Physiology, Pauwelsstr. 30, D-52057, Aachen, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 163-164/1(1996-03-01), 181-183 0300-8177 163-164:1<181 1996 163-164 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer