caa a22 4500 477120946 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248458 doi (NATIONALLICENCE)springer-10.1007/BF00248458 Pharmacology of endothelins: vascular preparations for studying ETA and ETB receptors [Elektronische Daten] [G. Caló, J. Gratton, S. Télémaque, P. D'Orléans-Juste, D. Regoli] Three rabbit vessels, the carotid and pulmonary arteries and the jugular vein were investigated to identify vascular monoreceptor systems (either ETA or ETB) to be used in structure-activity studies on endothelins and their antagonists. The RbCA has been found to behave as a monoreceptor ETA preparation, since it shows much greater sensitivity to ET-1 than to ET-3 and is insensitive to IRL 1620. The contractile response of the RbCA to ET-1 is reduced in the presence of BQ-123 but is not influenced by BQ-788. The RbPA behaves as a pure ETB system when stimulated with the ETB selective agonist IRL 1620. The contractile effect of IRL 1620 is reduced in the presence of BQ-788 but is not influenced by BQ-123. The RbJV responds to ETA and to IRL 1620 with contractions that are reduced by both BQ-123 and BQ-788, respectively. The RbJV appears to be a mixed ETA and ETB system in which the two functional sites play an equivalent role in the stimulatory contractile response. Thus, contractile ETA and ETB receptors have been found in arterial and venous vessels of the rabbit and some of these vessels provide sensitive and selective (either ETA or ETB) preparations that appear to be adequate for pharmacological studies on ET receptor agonists or antagonists. Kluwer Academic Publishers, 1996 endothelin nationallicence receptors nationallicence vascular smooth muscle nationallicence rabbit nationallicence ETA and ETB selective antagonists nationallicence Caló G. Institute of Pharmacology, University of Ferrara, via Fossato di Mortara 17-19, 44100, Ferrara, Italy aut Gratton J. Department of Pharmacology, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Télémaque S. Howard Hughes Medical Institute, UT Southwestern Medical Center, 5323 Herry Hines Boulevard, 75235-9050, Dallas, Texas, USA aut D'Orléans-Juste P. Department of Pharmacology, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Regoli D. Institute of Pharmacology, University of Ferrara, via Fossato di Mortara 17-19, 44100, Ferrara, Italy aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 31-37 0300-8177 154:1<31 1996 154 11010 https://doi.org/10.1007/BF00248458 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248458 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caló G. Institute of Pharmacology, University of Ferrara, via Fossato di Mortara 17-19, 44100, Ferrara, Italy aut NATIONALLICENCE 700 1- Gratton J. Department of Pharmacology, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Télémaque S. Howard Hughes Medical Institute, UT Southwestern Medical Center, 5323 Herry Hines Boulevard, 75235-9050, Dallas, Texas, USA aut NATIONALLICENCE 700 1- D'Orléans-Juste P. Department of Pharmacology, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Regoli D. Institute of Pharmacology, University of Ferrara, via Fossato di Mortara 17-19, 44100, Ferrara, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 31-37 0300-8177 154:1<31 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer