caa a22 4500 477120989 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248455 doi (NATIONALLICENCE)springer-10.1007/BF00248455 Biochemical and immunological identification and enrichment of poly(A) polymerase from human thymus [Elektronische Daten] [Christina Kyriakopoulou, Chris Tsiapalis, Maria Havredaki] Human thymus poly(A) polymerase (EC 2.7.7.19) activity has been investigated using poly(A) and oligo(A) as initiators. All obtained fractions reveal more than one polypeptide as detected by immunoblotting after SDS-PAGE. In addition to the homogeneously purified (Tsiapalis et al., J Biol Chem 250: 4486-1496, 1975 and Wahle, J Biol Chem 266: 3131-3139, 1991), about 60 kDa polypeptide, a larger polypeptide, about 80 kDa, that comigrates in the region of poly(A) polymerase activity was detected, enriched and partially characterized; it appears having similar size with bovine poly(A) polymerase cloned in E. coli. Polyclonal antiserum produced against recombinant bovine poly(A) polymerase reacts more efficiently with the about 80 kDa polypeptide upon immunoblotting, and can precipitate the poly(A) polymerase activity. This enzyme form, from human tissue, is novel in terms of size and may reflect intact or physiological form of poly(A) polymerase in human thymus, and supports and substantiates recent reports on the enzyme from other sources. Kluwer Academic Publishers, 1996 human thymus poly(A) polymerase nationallicence polyadenylation nationallicence immunological characterization nationallicence Kyriakopoulou Christina Institute of Biology, NCSR ‘Demokritos' Aghia Paraskevi Attikis 153 10, Greece aut Tsiapalis Chris Department of Biochemistry, Papanikolaou Research Center, 171 Alexandras Avenue, 115 22, Athens, Greece aut Havredaki Maria Institute of Biology, NCSR ‘Demokritos' Aghia Paraskevi Attikis 153 10, Greece aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 9-16 0300-8177 154:1<9 1996 154 11010 https://doi.org/10.1007/BF00248455 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248455 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kyriakopoulou Christina Institute of Biology, NCSR ‘Demokritos' Aghia Paraskevi Attikis 153 10, Greece aut NATIONALLICENCE 700 1- Tsiapalis Chris Department of Biochemistry, Papanikolaou Research Center, 171 Alexandras Avenue, 115 22, Athens, Greece aut NATIONALLICENCE 700 1- Havredaki Maria Institute of Biology, NCSR ‘Demokritos' Aghia Paraskevi Attikis 153 10, Greece aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 9-16 0300-8177 154:1<9 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer