caa a22 4500 477121004 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248464 doi (NATIONALLICENCE)springer-10.1007/BF00248464 Vitamin A supplementation inhibits chemiluminescence and lipid peroxidation in isolated rat liver microsomes and mitochondria [Elektronische Daten] [A. Palacios, V. Piergiacomi, A. Catalá] In the present study we investigated if administration of vitamin A could protect rat liver microsomes and mitochondria from in vitro peroxidation. Appreciable decrease of chemiluminescence and lipid peroxidation was measured in microsomal membranes from rats receiving vitamin A, with respect to control animals. In membranes derived from control animals, the fatty acid composition was profoundly modified when subjected to in vitro peroxidation mediated by ascorbate-Fe++, with a considerable decrease of 20:4 n6 and 22:6 n3 in mitochondria and 18:2 n6 and 20:4 n6 in microsomes. As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in supplemented animals than in control group when both kind of membranes were analyzed. These changes were less pronounced in membranes derived from rats receiving vitamin A. These results are in agreement with previous results that indicated that vitamin A may act as an antioxidant protecting membranes from deleterious effects. Kluwer Academic Publishers, 1996 Vitamin A nationallicence rat liver nationallicence microsomes nationallicence mitochondria nationallicence peroxidation chemiluminescence nationallicence BHT : butylated hydroxytoluene nationallicence BSA : bovine serum albumin nationallicence CL : chemiluminescence nationallicence PI : peroxidizability index nationallicence Palacios A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut Piergiacomi V. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut Catalá A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 77-82 0300-8177 154:1<77 1996 154 11010 https://doi.org/10.1007/BF00248464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palacios A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Piergiacomi V. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Catalá A. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900, La Plata, Argentina aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 77-82 0300-8177 154:1<77 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer