caa a22 4500 477121012 CHVBK 20180405111636.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248454 doi (NATIONALLICENCE)springer-10.1007/BF00248454 Decrease in the glycosaminoglycan content in the skin of diabetic rats. The role of IGF-I, IGF-binding proteins and proteolytic activity [Elektronische Daten] [Marzanna Cechowska-Pasko, Jerzy Pałka, Edward Bańkowski] The results of our previous studies demonstrated that acute streptozotocin-induced diabetes in rats evoked a decrease in skin collagen content with little effect on glycosaminoglycans (GAG) content. In our present study we employed the model of chronic diabetes in order to check its effect on skin GAG content. It was found that the skin of diabetic rats showed a significant decrease in almost all the investigated GAGs by 50-70%. The decrease in heparan sulfate content was slight and statistically insignificant. We sought to determine whether the insulin-like growth factor-I (IGF-1) and IGF-binding proteins (IGF-BPs) levels are altered in animals with experimental diabetes and might contribute to the decrease in tissue GAG content. Circulating IGF-I level was found to be reduced in animals with diabetes and significant changes in serum IGF-BPs were observed. The amount of high molecular weight binding proteins (HMW-BPs) was decreased and the content of low molecular weight binding proteins (LMW-BPs), known as IGF-I inactivating substances, markedly increased. Furthermore, diabetic rats demonstrated an increase of skin proteolytic activity. We conclude that the decrease of GAG content in the skin of diabetic rats is a result of three co-existing phenomena: decreased circulating IGFI level, increased plasma content of LMW BPs and increased proteolytic activity of the skin. Kluwer Academic Publishers, 1996 diabetes nationallicence glycosaminoglycan nationallicence IGF-I nationallicence proteolysis nationallicence skin nationallicence Cechowska-Pasko Marzanna Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut Pałka Jerzy Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut Bańkowski Edward Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 1-8 0300-8177 154:1<1 1996 154 11010 https://doi.org/10.1007/BF00248454 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248454 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cechowska-Pasko Marzanna Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut NATIONALLICENCE 700 1- Pałka Jerzy Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut NATIONALLICENCE 700 1- Bańkowski Edward Department of Biochemistry, Medical Academy of Białystok, 15-230, Białystok-8, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 1-8 0300-8177 154:1<1 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer