caa a22 4500 477121039 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248465 doi (NATIONALLICENCE)springer-10.1007/BF00248465 Fructose-1,6-bisphosphate as a metabolic substrate in hog ileum smooth muscle during hypoxial [Elektronische Daten] [Timothy Juergens, Christopher Hardin] Exogenously applied fructose-1,6-bisphosphate has been reported to be effective in preventing some damage to the small intestine during ischemia. To determine whether exogenously applied fructose-1,6-bisphosphate protects ileum smooth muscle from damage from hypoxia and from reoxygenation, we examined the effect of fructose-1,6-bisphosphate on the ability of hog ileum smooth muscle to maintain isometric force during hypoxia and to generate isometric force after reoxygenation in the presence of 5 mM glucose. After 180 min of hypoxia, tissues incubated with 20 mM fructose-1,6-bisphosphate maintained significantly greater levels of isometric force than tissues incubated in the absence of exogenous substrate (23% of pre-hypoxia force compared to 16%). During the first contraction following reoxygenation there was a significantly greater force generation in tissues incubated with 20 mM fructose-1,6-bisphosphate during the hypoxia period compared to tissues with no exogenous substrate included during the hypoxia period (29% of pre-hypoxia force compared to 19%). However, glucose always was a better metabolic substrate compared to fructose-1,6-bisphosphate under all experimental conditions. The presence of fructose-1,6-bisphosphate during hypoxia likely improved tissue function by fructose-1,6-bisphosphate entering the cells and acting as a glycolytic intermediate, since during a 120 min period of hypoxia, unmounted ileum smooth muscle metabolized 1,6-13C-fructose-1,6-bisphosphate to 3-13C-lactate. This conversion of 1,6-13C-fructose-1,6-bisphosphate to 3-13C-lactate was inhibited by the addition of 1 mM iodoacetic acid, a glycolytic inhibitor. We conclude that exogenously provided fructose-1,6-bisphosphate does provide modest protection of ileum smooth muscle from hypoxic damage by functioning as a glycolytic intermediate and improving the cellular energy state. Kluwer Academic Publishers, 1996 glycolysis nationallicence 13C-NMR nationallicence ischemia nationallicence hypoxia nationallicence smooth muscle nationallicence contraction nationallicence Juergens Timothy Department of Physiology, University of Missouri, 65212, Columbia, MO, USA aut Hardin Christopher Department of Physiology, University of Missouri, 65212, Columbia, MO, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 83-93 0300-8177 154:1<83 1996 154 11010 https://doi.org/10.1007/BF00248465 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juergens Timothy Department of Physiology, University of Missouri, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Hardin Christopher Department of Physiology, University of Missouri, 65212, Columbia, MO, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 83-93 0300-8177 154:1<83 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer