caa a22 4500 477121047 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00248462 doi (NATIONALLICENCE)springer-10.1007/BF00248462 Torsionally-strained DNA and intermolecular purine-purine-pyrimidine triple-helix formation [Elektronische Daten] [Marco Musso, Michael Van Dyke] A potentially powerful pharmacological approach to modulating the expression of specific, disease-related genes involves the inhibition of transcription factor binding to promoter or enhancer elements through oligonucleotide-mediated triple-helix formation. In vivo, the typical target for intermolecular triplex formation would most likely be torsionally-strained rather than relaxed duplex DNA. To determine the effects of strained DNA on triplex formation, we investigated the interactions between a G/T rich oligonucleotide and both supercoiled and relaxed plasmid DNA using a restriction endonuclease protection assay. Both the kinetics of formation and dissociation of purine-motif triplexes were unaffected by the conformational state of the duplex DNA. Similarly, the topological state of the plasmid targets was not affected by triplex formation. Taken together, these observations suggest that stable intermolecular triplexes can form in vivo under conditions of moderate torsional strain. Kluwer Academic Publishers, 1996 gene expression nationallicence regulatory elements nationallicence plasmid nationallicence oligonucleotides nationallicence Musso Marco Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas, USA aut Van Dyke Michael Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 65-70 0300-8177 154:1<65 1996 154 11010 https://doi.org/10.1007/BF00248462 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00248462 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Musso Marco Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Van Dyke Michael Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 154/1(1996-03-01), 65-70 0300-8177 154:1<65 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer