caa a22 4500 477121055 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226786 doi (NATIONALLICENCE)springer-10.1007/BF00226786 Identification of guanine nucleotide binding regulatory proteins in bovine tracheal smooth muscle [Elektronische Daten] [Sharmishtha Joshi, Worku Abebe, Devendra Agrawal] The identity of G-proteins in airway smooth muscle is not well elucidated. In the present study, by immunoblotting using AS/7 antibody specific for Giα−1/2, EC/2 antibody specific for Giα−3 and RM/1 antibody specific for GSa, we identified, respectively, Mr 39, 41, 46 and 52 KDa, Mr 41 and 43 KDa, and Mr 43 and 46 KDa polypeptides of conventional (heterotrimeric) G-proteins in purified membranes of bovine tracheal smooth muscle. The identity of the Mr 41, 43 and 52 KDa Giα, and the Mr 43 and 46 Gsα was also confirmed by ADP-ribosylation with pertussis and cholera toxins, respectively. In addition, the common antibody (AG/1) for both Giα and Gsα revealed the presence of all the above polypeptides, except the Mr 52 KDa band. By nitrocellulose blot overlay with [35S]GTPγS, we also detected seven low molecular weight GTP-binding proteins of Mr 18-30 KDa in the bovine tracheal smooth muscle. Photoaffinity crosslinking of [α-32P]GTP demonstrated the presence of high molecular GTP-binding proteins of Mr 55, 75 and 110 KDa. It is concluded that plasma membranes of bovine tracheal smooth muscle contain various types of conventional, low molecular weight and high molecular weight G-proteins. This warrantes further attention to elucidate the functional roles of G-proteins in airway smooth muscle. Kluwer Academic Publishers, 1996 G-proteins nationallicence airway smooth muscle nationallicence ADP-ribosylation nationallicence immunoblotting nationallicence photolabeling nationallicence nitrocellulose blot overlay nationallicence Joshi Sharmishtha Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut Abebe Worku Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut Agrawal Devendra Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 179-184 0300-8177 154:2<179 1996 154 11010 https://doi.org/10.1007/BF00226786 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226786 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joshi Sharmishtha Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut NATIONALLICENCE 700 1- Abebe Worku Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut NATIONALLICENCE 700 1- Agrawal Devendra Departments of Internal Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, NE, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 179-184 0300-8177 154:2<179 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer