caa a22 4500 47712108X CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226779 doi (NATIONALLICENCE)springer-10.1007/BF00226779 Implication of the nucleus in excitation contraction coupling of heart cells [Elektronische Daten] [Ghassan Bkaily, Nadine Gros-Louis, Radha Naik, Doris Jaalouk, Pierre Pothier] In the present study, Fluo-3 Ca2+ measurement and confocal microscopy techniques were used in order to localize cytosolic [ ]c and nuclear [ ]n free Ca2+ distribution in resting and spontaneously contracting single heart cells from 10-day-old chick embryos. In resting single cells, the concentration of Ca2+ in the cytoplasm was lower than that in the nucleus. Increasing cytosolic free Ca2+ from 100-1600 nM gradually increased [Ca2+]n with a maximum capacity near 1200 nM. Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes. Calcium waves in spontaneously contracting cells were found to spread from one cell to the next with the nucleus acting as a fluorescent beacon in which Ca2+ levels remained elevated for several milliseconds even after cytosolic Ca2+ had returned to near basal values. These results strongly suggest that the nucleus plays a negative and positive feedback role in controlling cytosolic free Ca2+ concentration during excitation-contraction coupling in heart cells. Kluwer Academic Publishers, 1996 heart cells nationallicence nucleus nationallicence calcium nationallicence R-type channel nationallicence excitation-contraction coupling nationallicence pacemaker activity nationallicence Fura-2 nationallicence Fluo-3 nationallicence confocal microscopy nationallicence Bkaily Ghassan MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Gros-Louis Nadine MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Naik Radha MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Jaalouk Doris MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Pothier Pierre MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 113-121 0300-8177 154:2<113 1996 154 11010 https://doi.org/10.1007/BF00226779 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226779 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bkaily Ghassan MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Gros-Louis Nadine MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Naik Radha MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Jaalouk Doris MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 700 1- Pothier Pierre MRCC Group in Immuno-Cardiovascular Interactions, Department of Physiology and Biophysics, Faculty of Medicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Quebec, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 113-121 0300-8177 154:2<113 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer