caa a22 4500 477121101 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226776 doi (NATIONALLICENCE)springer-10.1007/BF00226776 The possible relevance of autoxidative glycosylation in glucose mediated alterations of proteins: An in vitro study on myofibrillar proteins [Elektronische Daten] [G.B Sajith Lal, Pandarinathan Chithra, Gowri Chandrakasan] The present work was carried out to examine the role of glycation and transition metal catalysed autoxidation of sugars in glucose-mediated alterations of myofibrillar proteins. Myofibrils were prepared from rat skeletal muscle and incubated with 1) sugar alone 2) sugar and micromolar concentrations of transition metals (Cu2+ or Fe3+) 3) transition metals alone and the control remained without sugar or transition metals. A significant increase in extent of glycation and decrease in ATPase activity of myofibrils incubated under autoxidative conditions were observed over the other three incubations. Reducing agent 2-mercaptoethanol was highly effective in preventing the alterations induced by glucoxidation, compared to EDTA and aminoguanidine, suggesting the involvement of thiol group oxidation in the reduced function of the protein. Free radical scavengers like catalase, benzoic acid and mannitol were also effective in preventing glucose mediated alterations. Although a high concentration of glucose alone has an insignificant effect on myofibrils in vitro, the results from the present work suggest that glucose in combination with transition metals could lead to functional alterations of myofibrils, and this process by generating free radicals may contribute to the overall complications of diabetes and aging. Kluwer Academic Publishers, 1996 glycation nationallicence autoxidation nationallicence myofibrils nationallicence free radicals nationallicence Sajith Lal G.B. Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut Chithra Pandarinathan Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut Chandrakasan Gowri Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 95-100 0300-8177 154:2<95 1996 154 11010 https://doi.org/10.1007/BF00226776 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226776 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sajith Lal G.B. Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut NATIONALLICENCE 700 1- Chithra Pandarinathan Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut NATIONALLICENCE 700 1- Chandrakasan Gowri Department of Biochemistry, Central Leather Research Institute Adyar, 600 020, Madras, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 95-100 0300-8177 154:2<95 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer