caa a22 4500 47712111X CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226782 doi (NATIONALLICENCE)springer-10.1007/BF00226782 Non-proline-dependent protein kinases phosphorylate several sites found in tau from Alzheimer disease brain [Elektronische Daten] [Toolsee Singh, Tanweer Zaidi, Inge Grundke-Iqbal, Khalid Iqbal] Of 21 phosphorylation sites identified in PHF-tau 11 are on ser/thr-X motifs and are probably phosphorylated by non-proline-dependent protein kinases (non-PDPKs). The identities of the non-PDPKs and how they interact to hyperphosphorylate PHF-tau are still unclear. In a previous study we have shown that the rate of phosphorylation of human tau 39 by a PDPK (GSK-3) was increased several fold if tau were first prephosphorylated by non-PDPKs (Singh et al., FEBS Lett 358: 267-272, 1995). In this study we have examined how the specificity of a non-PDPK for different sites on human tau 39 is modulated when tau is prephosphorylated by other non-PDPKs (A-kinase, C-kinase, CK-1, CaM kinase II) as well as a PDPK (GSK-3). We found that the rate of phosphorylation of tau 39 by a non-PDPK can be stimulated if tau were first prephosphorylated by other non-PDPKs. Of the four non-PDPKs only CK-1 can phosphorylate sites (thr 231, ser 396, ser 404) known to be present in PHF-tau. Further, these sites were phosphorylated more rapidly and to a greater extent by CK-1 if tau 39 were first prephosphorylated by A-kinase, CaM kinase II or GSK-3. These results suggest that the site specificities of the non-PDPKs that participate in PHF-tau hyperphosphorylation can be modulated at the substrate level by the phosphorylation state of tau. Kluwer Academic Publishers, 1996 GSK-3 nationallicence tau protein nationallicence protein kinases nationallicence Alzheimer disease nationallicence paired helical filaments nationallicence microtubules nationallicence PHF : paired helical filaments nationallicence A-kinase : cyclic AMP-dependent protein kinase nationallicence CaM kinase II : calcium/calmodulin-dependent protein kinase II nationallicence C-kinase : calcium/phospholipid-dependent protein kinase nationallicence CK-1 : casein kinase-1 nationallicence CK-2 : casein kinase-2 nationallicence GSK-3 : glycogen synthase kinase-3 nationallicence MAP kinase : mitogen-activated protein kinase nationallicence PDPK : proline-dependent protein kinase nationallicence Singh Toolsee New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut Zaidi Tanweer New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut Grundke-Iqbal Inge New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut Iqbal Khalid New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 143-151 0300-8177 154:2<143 1996 154 11010 https://doi.org/10.1007/BF00226782 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226782 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Toolsee New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut NATIONALLICENCE 700 1- Zaidi Tanweer New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut NATIONALLICENCE 700 1- Grundke-Iqbal Inge New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut NATIONALLICENCE 700 1- Iqbal Khalid New York State Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 143-151 0300-8177 154:2<143 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer