caa a22 4500 477121128 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226785 doi (NATIONALLICENCE)springer-10.1007/BF00226785 Effects of angiotensin II and aldosterone on collagen gene expression and protein turnover in cardiac fibroblasts [Elektronische Daten] [Guoping Zhou, Jagannadha Kandala, Suresh Tyagi, Laxmansa Katwa, Karl Weber] Earlier studies have demonstrated angiotensin II (AngII) and aldosterone (ALDO) each augment cultured adult rat cardiac fibroblast (CFb) collagen synthesis. Whether this involves type I collagen, the major structural protein of the myocardium, and represents a transcriptional event, is uncertain. Accordingly, the influence of AngII and ALDO on transcription and synthesis of fibrillar collagen and on collagenolytic activity was examined in cultured CFb maintained in serum-deprived media. Using concentrations for AngII (10−7 M) or ALDO (10−9 M), shown to influence collagen turnover in these cells, we found: a) total collagen synthesis was significantly (p < 0.05) increased (5.4 ± 0.41 and 4.8 ± 0.37 vs. control 3.1 ± 0.55); b) type I collagen production (6590 ± 710 and 6150 ± 410 vs. control 4700 ± 490 ng/mL) in the medium were significantly (p < 0.01) increased; c) type I collagen mRNA expression was also significantly (p < 0.01) increased by AngII (2.0 fold) and ALDO (1.8 fold) compared with control; d) AngII, but not ALDO, significantly (p < 0.05) decreased collagenolytic activity (0.5 fold) compared with control. Thus, AngII and ALDO each increase CFb type I collagen synthesis at the level of transcription and protein synthesis and AngII, but not ALDO, alters collagenolytic activity. Such hormonally mediated alterations in CFb collagen turnover may contribute to the adverse accumulation of fibrillar collagen found in the myocardium in various disease states, where circulating AngII and/or ALDO are increased. Kluwer Academic Publishers, 1996 collagen synthesis nationallicence Type I collagen nationallicence collagen degradation nationallicence cardiac fibroblast nationallicence angiotensin II nationallicence aldosterone nationallicence Zhou Guoping Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut Kandala Jagannadha Department of Molecular Microbiology & Immunology, University of Missouri-Columbia, 65212, Columbia, MO, USA aut Tyagi Suresh Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut Katwa Laxmansa Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut Weber Karl Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 171-178 0300-8177 154:2<171 1996 154 11010 https://doi.org/10.1007/BF00226785 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226785 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Guoping Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Kandala Jagannadha Department of Molecular Microbiology & Immunology, University of Missouri-Columbia, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Tyagi Suresh Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Katwa Laxmansa Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Weber Karl Division of Cardiology, University of Missouri-Columbia, MA 432 Medical Science Building, 65212, Columbia, MO, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 171-178 0300-8177 154:2<171 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer