caa a22 4500 477121144 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226777 doi (NATIONALLICENCE)springer-10.1007/BF00226777 Ethanol-dependent oxygen consumption and acetaldehyde formation during vanadyl oxidation by H2O2 [Elektronische Daten] [H. Ravishankar, Aparna Rao, T. Ramasarma] Sequential addition of vanadyl sulfate to a phosphate-buffered solution of H2O2 released oxygen only after the second batch of vanadyl. Ethanol added to such reaction mixtures progressively decreased oxygen release and increased oxygen consumption during oxidation of vanadyl by H2O2. Inclusion of ethanol after any of the three batches of vanadyl resulted in varying amounts of oxygen consumption, a property also shared by other alcohols (methanol, propanol and octanol). On increasing the concentration of ethanol, vanadyl sulfate or H2O2, both oxygen consumption and acetaldehyde formation increased progressively. Formation of acetaldehyde decreased with increase in the ratio of vanadyl:H2O2 above 2:1 and was undetectable with ethanol at 0.1 mM. The reaction mixture which was acidic in the absence of phosphate buffer (pH 7.0), released oxygen immediately after the first addition of vanadyl and also in presence of ethanol soon after initial rapid consumption of oxygen, with no accompanying acetaldehyde formation. The results underscore the importance of some vanadium complexes formed during vanadyl oxidation in the accompanying oxygen-transfer reactions. Kluwer Academic Publishers, 1996 vanadyl oxidation nationallicence ethanol-stimulated oxygen consumption nationallicence acetaldehyde formation nationallicence Ravishankar H. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut Rao Aparna Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut Ramasarma T. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 101-106 0300-8177 154:2<101 1996 154 11010 https://doi.org/10.1007/BF00226777 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226777 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ravishankar H. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut NATIONALLICENCE 700 1- Rao Aparna Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut NATIONALLICENCE 700 1- Ramasarma T. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 101-106 0300-8177 154:2<101 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer