caa a22 4500 477121152 CHVBK 20180405111637.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00226778 doi (NATIONALLICENCE)springer-10.1007/BF00226778 Influence of thyroid hormones on the human ATP synthase β-subunit gene promoter [Elektronische Daten] [Immaculada Martin, Josep Villena, Marta Giralt, Roser Iglesias, Teresa Mampel, Octavi Vińas, Francesc Villarroya] The action of thyroid hormones on the expression of the mitochondrial ATP synthase β-subunit gene (ATPsynβ) is controversial. We detected a binding site for the thyroid hormone receptor between-366 and-380 in the human ATPsynβ gene by DNase I footprint analysis and band-shift assays. However, expression vectors in which the chloramphenicol acetyl transferase (CAT) reporter gene is driven by the 5′ upstream region of ATPsynβ gene were unresponsive to T3 when transiently transfected to HepG2 or GH4C1 cells. CAT constructs driven by the rat phosphoenolpyruvate carboxykinase (PEPCK) or the growth hormone (GH) promoters were stimulated several fold by T3 in parallel experiments. It is proposed that the biological effects of thyroid hormones on the ATPsynβ expression occur through indirect mechanisms. Kluwer Academic Publishers, 1996 ATP synthase β-subunit gene nationallicence mitochondria nationallicence thyroid hormone nationallicence (human) nationallicence Martin Immaculada Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Villena Josep Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Giralt Marta Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Iglesias Roser Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Mampel Teresa Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Vińas Octavi Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Villarroya Francesc Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl 154/2(1996-09-01), 107-111 0300-8177 154:2<107 1996 154 11010 https://doi.org/10.1007/BF00226778 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226778 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin Immaculada Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Villena Josep Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Giralt Marta Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Iglesias Roser Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Mampel Teresa Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Vińas Octavi Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 700 1- Villarroya Francesc Unitat de Bioquímica i Biologia Molecular B, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; gopher//gopher.wkap.nl/ 154/2(1996-09-01), 107-111 0300-8177 154:2<107 1996 154 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer