caa a22 4500 47712528X CHVBK 20180405111647.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1007/BF02505320 doi (NATIONALLICENCE)springer-10.1007/BF02505320 Sperm count in ejaculates and after sperm selection with discontinuous percoll gradient centrifugation technique, as a prognostic index of IVF outcome [Elektronische Daten] [M. Hammadeh, M. Stieber, G. Haidl, W. Schmidt] Success of IVF with low sperm count will depend on the retrieval of the maximum number of normal motile sperms, and this makes the selection of an appropriate technique critical. Various in vitro methods have been developed for selecting human sperm cells. Sperm selection using percoll gradient has been reported to yield upto 60% motile and morphologically normal spermatozoa from a normal semen sample. In this study, we have attempted to determine a possible relationship between sperm count in ejaculates before and after selection with percoll gradient centrifugation on one hand, and fertilization, cleavage and pregnancy rates in an IVF program on the other. With increased sperm count at the time of IVF treatment using selected sperm, we observed higher fertilization, cleavage and gestation rates. In conclusion, sperm concentration before and after selection with percoll may be considered a prognostic parameter for the determination of fertilization potential and pregnancy rates in an IVF program. Springer-Verlag, 1997 Sperm density before/after selection nationallicence In-vitro fertilization nationallicence Discontinous percoll gradient centrifugation nationallicence Pregnancy nationallicence Hammadeh M. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut Stieber M. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut Haidl G. Department of Dermatology and Venereology, Rheinische Friedrich-Wilhelms-University, Bonn, Germany aut Schmidt W. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 259/3(1997-04-01), 125-131 0932-0067 259:3<125 1997 259 404 https://doi.org/10.1007/BF02505320 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02505320 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hammadeh M. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Stieber M. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Haidl G. Department of Dermatology and Venereology, Rheinische Friedrich-Wilhelms-University, Bonn, Germany aut NATIONALLICENCE 700 1- Schmidt W. Department of Obstetrics and Gynaecology, University of Saarland, D-66421, Homburg/Saar, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 259/3(1997-04-01), 125-131 0932-0067 259:3<125 1997 259 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer