caa a22 4500 477125301 CHVBK 20180405111647.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1007/BF02505318 doi (NATIONALLICENCE)springer-10.1007/BF02505318 Effect of peritoneal fluid from patients with mild and severe endometriosis on endometrial stromal cell proliferation [Elektronische Daten] [Gabriela Meresman, Rosa Barañao, A. Tenenbaum, J. Singla, N. Neuspiller, Lia Rumi] The aim of this study was to evaluate and compare the mitogenic effect of peritoneal fluid (PF) from women with mild and severe endometriosis on the endometrial stromal cell proliferation. Increasing concentrations of PF from women with and without mild or severe endometriosis were added to primary endometrial stromal cell cultures and3H-thymidine incorporation was used to assess DNA synthesis in these cultures. PF from women with mild endometriosis induced a statistically significant dose-dependent increase in stromal cell thymidine uptake ranged from 5.8 to 14.5 fold, whereas PF from women with severe endometriosis produced an average 51% inhibition of stromal cell proliferation of compared with cells exposed to non-endometriosis PF or exposed to nutrient medium supplemented with 2.5% calf serum alone. PF samples from patients with stage I endometriosis induced a statistically dose-dependent increase in stromal cell proliferation, whereas PF from patients with stage IV endometriosis caused a significant inhibition. Springer-Verlag, 1997 Endometriosis nationallicence Peritoneal fluid nationallicence Endometrial stromal cells nationallicence Meresman Gabriela Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut Barañao Rosa Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut Tenenbaum A. Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut Singla J. Fecunditas, Larrea 790, Buenos Aires, Argentina aut Neuspiller N. Fecunditas, Larrea 790, Buenos Aires, Argentina aut Rumi Lia Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut Archives of Gynecology and Obstetrics Springer-Verlag 259/3(1997-04-01), 109-115 0932-0067 259:3<109 1997 259 404 https://doi.org/10.1007/BF02505318 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02505318 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meresman Gabriela Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Barañao Rosa Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Tenenbaum A. Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Singla J. Fecunditas, Larrea 790, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Neuspiller N. Fecunditas, Larrea 790, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Rumi Lia Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 259/3(1997-04-01), 109-115 0932-0067 259:3<109 1997 259 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer