caa a22 4500 477125522 CHVBK 20180405111648.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1012043131081 doi (NATIONALLICENCE)springer-10.1023/A:1012043131081 Influence of Fluorescent Labelling of Polystyrene Particles on Phagocytic Uptake, Surface Hydrophobicity, and Plasma Protein Adsorption [Elektronische Daten] [Rainer Müller, Dörte Rühl, Martin Lück, Bernd.-R. Paulke] Purpose. To investigate the influence of fluorescent labelling of polystyrene particles on phagocytic uptake, surface hydrophobicity and protein adsorption. Methods. Phagocytic uptake was analysed using chemiluminescence. Hydrophobicity was quantified by adsorption measurements of a hydrophobic dye. Protein adsorption was evaluated by two-dimensional electrophoresis. Results. Commercially available fluorescently labelled particles showed marked differences when compared to unlabelled particles: phagocytic uptake and surface hydrophobicity of labelled particles were diminished. Also the plasma protein adsorption pattern was found to be different from the unlabelled particles: for example, the amount of fibrinogen adsorbed was strongly reduced on the labelled particles. On the other hand, some unknown proteins could be detected on the fluorescently marked particles. In contrast, plain polystyrene particles and labelled ones could be successfully synthesised by Paulke which did not show any considerable differences in phagocytic uptake, surface hydrophobicity and protein adsorption. Polysorbate 20 added as stabilizer to particle suspensions led to completely different behaviour of the particles: the particles showed altered protein adsorption patterns, dominated by immunoglobulins and especially by apolipoproteins. Furthermore, these particles were not phagocytized at all. Conclusions. Surface hydrophobicity and phagocytic uptake in vitro as well as the interactions with plasma proteins of commercially available polystyrene particles were strongly affected by fluorescent labelling. Particles synthesised by Paulke remained unchanged after labelling. The results show the importance of thorough surface characterization for using particles in test systems in vitro and in vivo. Plenum Publishing Corporation, 1997 polystyrene particles nationallicence fluorescent labelling nationallicence phagocytic uptake nationallicence hydrophobicity nationallicence protein adsorption nationallicence 2-D PAGE nationallicence Müller Rainer Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut Rühl Dörte Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut Lück Martin Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut Paulke Bernd.-R Max-Planck-Institute for Colloid and Interface Research (MPI), Kant-straβe 55, 14513, Teltow aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/1(1997-01-01), 18-24 0724-8741 14:1<18 1997 14 11095 https://doi.org/10.1023/A:1012043131081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012043131081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müller Rainer Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut NATIONALLICENCE 700 1- Rühl Dörte Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut NATIONALLICENCE 700 1- Lück Martin Department of Pharmaceutics, Biopharmaceutics & Biotechnology, The Free University of Berlin, Kelchstraβe 31, D-12169, Berlin aut NATIONALLICENCE 700 1- Paulke Bernd.-R Max-Planck-Institute for Colloid and Interface Research (MPI), Kant-straβe 55, 14513, Teltow aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/1(1997-01-01), 18-24 0724-8741 14:1<18 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer