caa a22 4500 477125557 CHVBK 20180405111648.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1012059501947 doi (NATIONALLICENCE)springer-10.1023/A:1012059501947 Interaction Between Polyalkylcyanoacrylate Nanoparticles and Peritoneal Macrophages: MTT Metabolism, NET Reduction, and NO Production [Elektronische Daten] [Teresa Cruz, Rogério Gaspar, Ana Donato, Celeste Lopes] Purpose. The nature of interactions between macrophages and drug carriers is of primordial importance either in the design of more effective therapeutic strategies for macrophage-associated pathogenesis or in establishing new approaches for pharmacological action avoiding macrophages. Methods. Polyalkylcyanoacrylate nanoparticles (PMCA, PECA, PBCA and PIBCA nanoparticles) were assayed for their toxicity on peritoneal resident and thioglycolate-elicited macrophages. Cellular viability was assessed by MTT tetrazolium salt assay, oxidative burst by NBT reduction and NO production by nitrite evaluation. Results. The nanoparticles tested led to cellular morphological modifications and induced toxicity in both types of macrophages in culture. The polyalkylcyanoacrylate nanoparticles uptake by peritoneal macrophages caused an increase in respiratory burst, as assessed by the NBT reduction assay, and induced the release of soluble toxic factors to the culture medium. The association of LPS with the PMCA nanoparticles significantly stimulated the production of nitric oxide (NO) by resident macrophages. In contrast, the association of PBCA nanoparticles with LPS does not increase the nitrite production as compared with LPS alone, which may be due to a different physico-chemical interaction between LPS and the two types of polymers. Conclusions. In cultured mice peritoneal macrophages, nanoparticles of PACA induce the production of oxygen reactive products, which cause changes in the cell metabolism of both resident and elicited macrophages. PMCA nanoparticles in association with LPS significantly increase the expression of the inducible isoform of nitric oxide synthase, leading to the release of large amount of NO, which may be highly cytotoxic to the cultured cells in the presence of peroxide generated from the oxidative burst. Plenum Publishing Corporation, 1997 nanoparticles nationallicence macrophages nationallicence cellular toxicity nationallicence NO production nationallicence NBT reduction nationallicence Cruz Teresa Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut Gaspar Rogério Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut Donato Ana Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut Lopes Celeste Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/1(1997-01-01), 73-79 0724-8741 14:1<73 1997 14 11095 https://doi.org/10.1023/A:1012059501947 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012059501947 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cruz Teresa Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut NATIONALLICENCE 700 1- Gaspar Rogério Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut NATIONALLICENCE 700 1- Donato Ana Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut NATIONALLICENCE 700 1- Lopes Celeste Faculdade de Farmácia and Centre de Neurociências, Universidade de Coimbra, Rua do Norte, 3000, Coimbra, Portugal aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/1(1997-01-01), 73-79 0724-8741 14:1<73 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer