caa a22 4500 477126111 CHVBK 20180405111650.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1012175428181 doi (NATIONALLICENCE)springer-10.1023/A:1012175428181 The Effect of Current on Skin Barrier Function In Vivo: Recovery Kinetics Post-Iontophoresis [Elektronische Daten] [Norris Turner, Yogeshvar Kalia, Richard Guy] Purpose. The objective of this study was to determine the extent to which current passage perturbed the skin's intrinsic permeability, and to quantify how quickly and to what extent the barrier properties recovered from the effects of iontophoresis. Methods. Laser scanning confocal microscopy (LSCM) and impedance spectroscopy (IS) were employed, respectively, to visualize and quantify the recovery kinetics. Results. LSCM images were obtained following passivecalcein diffusion through pre-iontophoresed HMS skin in vivothat had been allowed to recover for progressively longer periods of time. IS was used to quantify the rate and extent of skin permeability recovery following current pretreatment. Impedance spectra were recorded 0, 3, 5, 7, 9 and 18 hrs after current termination. Conclusions. Enhanced calcein permeability as assessed by confocal microscopy persisted for up to 24 hrs following current passage. Consistent with these LSCM findings, IS indicated that the time required for the impedance of hairless mouse skin to return to pre-iontophoresis levels (following 2-hr current passage at 0.5 mA/cm2) was at least 18 hrs. Plenum Publishing Corporation, 1997 iontophoresis nationallicence skin nationallicence barrier function nationallicence recovery kinetics nationallicence transport nationallicence impedance spectroscopy nationallicence Turner Norris Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut Kalia Yogeshvar Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut Guy Richard Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/9(1997-09-01), 1252-1257 0724-8741 14:9<1252 1997 14 11095 https://doi.org/10.1023/A:1012175428181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012175428181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turner Norris Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 700 1- Kalia Yogeshvar Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 700 1- Guy Richard Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California—San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/9(1997-09-01), 1252-1257 0724-8741 14:9<1252 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 222366346 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer