caa a22 4500 47712612X CHVBK 20180405111650.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1012154823638 doi (NATIONALLICENCE)springer-10.1023/A:1012154823638 Radiation Effects on Dopamine and Norepinephrine [Elektronische Daten] [J. Basly, I. Longy, M. Bernard] Purpose. Radiation sterilization is becoming increasingly popular for the sterilization of many pharmaceutical products. We have investigated the gamma radiation induced effects on dopamine and norepineprine by ESR spectroscopy. Results and Discussion. Equations to describe the evolution of the ESR curves versus doses and time of storage are presented. Linear regression is, for dopamine hydrochloride, applicable for doses ranging from 10 to 25 kGy. Since the radiation dose selected must always be based upon the bioburden of the products and the degree of sterility required, doses in the range 10−25 kGy could be investigated and linear regression would appear to be the least expensive route to follow and gives good results. The comportment of noradrenaline bitartrate is more complex and the use of linear regression would appear more hazardous especially for low doses. For doses higher than 25 kGy, a more general equation is required. Power function using only 2 parameters could give good results but must be validated. Decay kinetics for radicals versus storage were considered. Non-homogenous kinetics with time dependent rate constant and bi-exponential function appeared valid to reproduce the decay of radicals for, respectively, dopamine and norepinephrine. Conclusions. It is worth noting that, at present, ESR is the only technique which proved to be suitable for identification and quantification purposes in irradiated pharmaceuticals. Moreover, other features such as sensitivity, precision, ease and non-destructive readout make ESR superior to other proposed analytical techniques. Plenum Publishing Corporation, 1997 dopamine nationallicence norepinephrine nationallicence radiation treatment nationallicence ESR spectroscopy nationallicence dosimetry nationallicence storage nationallicence Basly J. Laboratoire de Chimie Analytique et Bromatologie, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut Longy I. Laboratoire de Chimie Analytique et Bromatologie, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut Bernard M. Laboratoire de Biophysique Pharmaceutique, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/9(1997-09-01), 1192-1196 0724-8741 14:9<1192 1997 14 11095 https://doi.org/10.1023/A:1012154823638 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012154823638 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Basly J. Laboratoire de Chimie Analytique et Bromatologie, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut NATIONALLICENCE 700 1- Longy I. Laboratoire de Chimie Analytique et Bromatologie, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut NATIONALLICENCE 700 1- Bernard M. Laboratoire de Biophysique Pharmaceutique, UFR de Pharmacie, Université de Limoges, 2 rue du docteur Marcland, 87025, Limoges Cedex, France aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/9(1997-09-01), 1192-1196 0724-8741 14:9<1192 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer