caa a22 4500 477126294 CHVBK 20180405111650.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1012104017761 doi (NATIONALLICENCE)springer-10.1023/A:1012104017761 Determination of Plasma Protein Adsorption on Magnetic Iron Oxides: Sample Preparation [Elektronische Daten] [Kai Thode, Martin Lück, Wolfhard Semmler, Rainer Müller, Mayk Kresse] Purpose. The purpose of this study was to investigate the influence of the sample preparation on the plasma protein adsorption pattern of polysaccharide-stabilized iron oxide particles by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Methods. The iron oxide particles were incubated in vitro in human plasma for five minutes. Thereafter, four different methods for particle recovery, including adsorbed proteins from surplus plasma, were investigated: centrifugation, magnetic separation, gel filtration and membrane-based static microfiltration. Adsorbed proteins were desorbed from the particle surfaces by surfactants and analyzed by 2-D PAGE, as described elsewhere (1,2). Results. All the techniques investigated were able to separate small-size iron oxides (approx. 110 nm) and adsorbed proteins from excess plasma. The gels obtained by the different separation procedures displayed almost identical adsorption patterns. Major proteins identified were: fibrinogen, IgG, albumin and an unclassified protein of about 70 kDa with a pI value of 6.5−7.5. Conclusions. Centrifugation was regarded as the most suitable separation method due to its speed and ease of use. In contrast to gel filtration, any washing media can be used. The magnetic separation process is restricted to particles with high inducible magnetic saturation, in particular, to iron oxides with overall sizes > 50 nm. Plenum Publishing Corporation, 1997 iron oxides nationallicence sample preparation nationallicence 2-D PAGE nationallicence plasma protein adsorption nationallicence Thode Kai Institut für Pharmazeutische Technologic, Biopharmazie und Biotechnologie, Freie Universität Berlin, Berlin, Germany aut Lück Martin Institut für Pharmazeutische Technologic, Biopharmazie und Biotechnologie, Freie Universität Berlin, Berlin, Germany aut Semmler Wolfhard Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut Müller Rainer Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut Kresse Mayk Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/7(1997-07-01), 905-910 0724-8741 14:7<905 1997 14 11095 https://doi.org/10.1023/A:1012104017761 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012104017761 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thode Kai Institut für Pharmazeutische Technologic, Biopharmazie und Biotechnologie, Freie Universität Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Lück Martin Institut für Pharmazeutische Technologic, Biopharmazie und Biotechnologie, Freie Universität Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Semmler Wolfhard Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut NATIONALLICENCE 700 1- Müller Rainer Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut NATIONALLICENCE 700 1- Kresse Mayk Institut fur Diagnostikforschung GmbH (IDF), Berlin, Germany aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/7(1997-07-01), 905-910 0724-8741 14:7<905 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer