caa a22 4500 477126367 CHVBK 20180405111651.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1012187414126 doi (NATIONALLICENCE)springer-10.1023/A:1012187414126 Cationic Lipid-Based Gene Delivery Systems: Pharmaceutical Perspectives [Elektronische Daten] [Ram Mahato, Alain Rolland, Eric Tomlinson] Gene delivery systems are designed to control the location of administered therapeutic genes within a patient's body. Successful in vivo gene transfer may require (i) the condensation of plasmid and its protection from nuclease degradation, (ii) cellular interaction and internalization of condensed plasmid, (iii) escape of plasmid from endosomes (if endocytosis is involved), and (iv) plasmid entry into cell nuclei. Expression plasmids encoding a therapeutic protein can be, for instance, complexed with cationic liposomes or micelles in order to achieve effective in vivo gene transfer. A thorough knowledge of pharmaceutics and drug delivery, bio-engineering, as well as cell and molecular biology is required to design optimal systems for gene therapy. This mini-review provides a critical discussion on cationic lipid-based gene delivery systems and their possible uses as pharmaceuticals. Plenum Publishing Corporation, 1997 non-viral gene delivery nationallicence plasmid nationallicence cationic liposomes nationallicence formulation nationallicence transfection nationallicence Mahato Ram GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut Rolland Alain GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut Tomlinson Eric GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/7(1997-07-01), 853-859 0724-8741 14:7<853 1997 14 11095 https://doi.org/10.1023/A:1012187414126 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012187414126 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahato Ram GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut NATIONALLICENCE 700 1- Rolland Alain GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut NATIONALLICENCE 700 1- Tomlinson Eric GENE MEDICINE, INC., 8301 New Trails Drive, 77381-4248, The Woodlands, Texas aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/7(1997-07-01), 853-859 0724-8741 14:7<853 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer