caa a22 4500 477126510 CHVBK 20180405111651.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1012140131757 doi (NATIONALLICENCE)springer-10.1023/A:1012140131757 The Effects of Hexetidine (Oraldene™) on the Adherence of Candida Albicans to Human Buccal Epithelial Cells In Vitro and Ex Vivo and on In Vitro Morphogenesis [Elektronische Daten] [David Jones, James McGovern, A. Woolfson, Sean Gorman] Purpose. This study reports the effects of hexetidine (Oraldene™) on two virulence attributes of Candida albicans, namely,in vitro and ex vivoadherence of yeast cells to buccal epithelial cells (EEC) and in vitro morphogenesis. Methods. The effects of hexetidine treatment of either yeast cells (stationary and exponential phases) or BEC on Candidal adherence, in terms of viable and non-viable adherent yeast cells, were evaluated using an acridine orange stain in conjunction with fluorescence microscopy. Ex vivoanti-adherence effects were determined by rinsing BEC in vivo with hexetidine (0.1%), removal of BEC after defined periods and inclusion in the adherence assay. The effects of hexetidine on morphogenesis were evaluated using light microscopy. Yeast cell viability following exposure to a range of concentration of hexetidine (0.005-0.1 % v/v) for defined periods was determined following serial dilution and enumeration on solid media. Results. Treatment of stationary and exponential phase yeast cells or BEC with hexetidine (0.1%) for a range of times (10−300 s) or, alternatively, with a range of concentrations of hexetidine (0.005−0.1 %) for a fixed time (30s) significantly decreased the resultant Candidal/ epithelial adhesion. No correlations were observed between reduced adherence and either time of treatment or hexetidine concentration. In vivotreatment of BEC with hexetidine (0.1%) for 30s resulted in prolonged and significant reductions in the ex vivo adherence of both viable and non-viable yeast cells for periods of up to (and including) four hours post-rinsing. Treatment of C. albicans blastospores with hexetidine (0.05, 0.1% v/v) for 10s and 30s totally inhibited Candida morphogenesis, whereas treatment with lower antiseptic concentrations significantly reduced the extent of Candida morphogenesis and the rate of hyphal development. The effects of hexetidine on yeast cell viability were both concentration and time-dependent. Conclusions. The reduced adherence of C. albicans to BEC and the modification or inhibition of morphogenesis following exposure to hexetidine suggests a clinical role for hexetidine in the prophylaxis of both superficial candidosis and the systemic complications resulting from invasion of sub-epithelial tissue. Plenum Publishing Corporation, 1997 Candida albicans nationallicence hexetidine nationallicence reduced adherence nationallicence in vitro nationallicence ex vivo nationallicence modified morphogenesis nationallicence Jones David School of Pharmacy, The Queen's University of Belfast, The Medical Biology Centre, 97, Lisburn Road, BT9 7BL, Belfast, Northern Ireland, United Kingdom aut McGovern James aut Woolfson A. aut Gorman Sean aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/12(1997-12-01), 1765-1771 0724-8741 14:12<1765 1997 14 11095 https://doi.org/10.1023/A:1012140131757 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012140131757 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones David School of Pharmacy, The Queen's University of Belfast, The Medical Biology Centre, 97, Lisburn Road, BT9 7BL, Belfast, Northern Ireland, United Kingdom aut NATIONALLICENCE 700 1- McGovern James aut NATIONALLICENCE 700 1- Woolfson A. aut NATIONALLICENCE 700 1- Gorman Sean aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/12(1997-12-01), 1765-1771 0724-8741 14:12<1765 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer