caa a22 4500 477126952 CHVBK 20180405111652.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1012046021028 doi (NATIONALLICENCE)springer-10.1023/A:1012046021028 Carrier-Mediated Transport of Monocarboxylic Acids in Primary Cultured Epithelial Cells from Rabbit Oral Mucosa [Elektronische Daten] [Naoki Utoguchi, Yoshiteru Watanabe, Takahisa Suzuki, Junko Maehara, Yoshiaki Matsumoto, Mitsuo Matsumoto] Purpose. Using primary cultured rabbit oral mucosal epithelial cells (ROEpi), we investigated whether carrier-mediated drug absorption via the oral mucosal route occurs. Methods. Oral mucosal epithelial cells were isolated from rabbit buccal mucosa and cultured on tissue culture plates. When the cells reached confluence, drug uptake experiments were performed. [14C]Benzoic acid or [14C] acetic acid was used as a marker for monocarboxylic acid carrier-mediated transport. Results. The uptake of [14C]benzoic acid by ROEpi occurred at a much lower rate at 4°C than at 37°C. The metabolic inhibitors, sodium azide and 2,4-dinitrophenol, significantly inhibited the uptake of [l4C]benzoic acid by ROEpi. Various monocarboxylic acids inhibited the uptake of [14C]benzoic acid or [14C]acetic acid by ROEpi, whereas dicarboxylic acids did not affect the uptake. Kinetic analysis using Lineweaver-Burk plots revealed that acetic acid competitively inhibited the uptake of [l4C]benzoic acid, and benzoic acid competitively inhibited the uptake of [l4C]acetic acid by ROEpi. Conclusions. There exists a carrier-mediated transport system for monocarboxylic acids in oral mucosal epithelial cells. Plenum Publishing Corporation, 1997 oral mucosa nationallicence carrier-mediated transport nationallicence epithelial cells nationallicence monocarboxylic acid nationallicence Utoguchi Naoki Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Watanabe Yoshiteru Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Suzuki Takahisa Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Maehara Junko Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Matsumoto Yoshiaki Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Matsumoto Mitsuo Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/3(1997-03-01), 320-324 0724-8741 14:3<320 1997 14 11095 https://doi.org/10.1023/A:1012046021028 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012046021028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Utoguchi Naoki Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 700 1- Watanabe Yoshiteru Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 700 1- Suzuki Takahisa Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 700 1- Maehara Junko Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsumoto Yoshiaki Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsumoto Mitsuo Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, 194, Tokyo, Japan aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/3(1997-03-01), 320-324 0724-8741 14:3<320 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer