caa a22 4500 477127061 CHVBK 20180405111652.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1012145410859 doi (NATIONALLICENCE)springer-10.1023/A:1012145410859 Lymphatic Uptake and Biodistribution of Liposomes After Subcutaneous Injection: III. Influence of Surface Modification with Poly(ethyleneglycol) [Elektronische Daten] [Christien Oussoren, Gert Storm] Purpose. The aim of the present paper was to assess the effect of inclusion of distearoylphosphatidylethanolamine-poly(ethyleneglycol) (DSPE-PEG) into liposomal bilayers on the lymphatic uptake and lymph node localization of liposomes after subcutaneous administration. Methods. [3H]-Cholesteryloleylether labeled liposomes of various composition and sizes were injected s.c. into the dorsal side of the foot of rats. At several time-points after injection, blood levels of liposomes were determined. Lymphatic uptake from the s.c. site of injection and lymph node localization in regional lymph nodes were determined at the end of the 52 h observation period. Results. The results demonstrate that inclusion of DSPE-PEG into several types of liposomes has only a modest effect on lymphatic uptake. Also lymph node localization is only slightly affected by PEG-mediated steric stabilization. Conclusions. Factors other than the presence of a steric barrier are more important in determining lymphatic uptake from the s.c. injection site. The observation that lymph node localization was only slightly affected by PEG-coating strongly suggests that macrophage uptake is not the only important mechanism of lymph node localization of s.c. administered liposomes. Plenum Publishing Corporation, 1997 liposomes nationallicence subcutaneous nationallicence lymphatic disposition nationallicence steric stabilization nationallicence Oussoren Christien Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, participant in Groningen Utrecht Institute for Drug Exploration (GUIDE), Faculty of Pharmacy, Utrecht, The Netherlands aut Storm Gert Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, participant in Groningen Utrecht Institute for Drug Exploration (GUIDE), Faculty of Pharmacy, Utrecht, The Netherlands aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/10(1997-10-01), 1479-1484 0724-8741 14:10<1479 1997 14 11095 https://doi.org/10.1023/A:1012145410859 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012145410859 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oussoren Christien Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, participant in Groningen Utrecht Institute for Drug Exploration (GUIDE), Faculty of Pharmacy, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Storm Gert Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, participant in Groningen Utrecht Institute for Drug Exploration (GUIDE), Faculty of Pharmacy, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/10(1997-10-01), 1479-1484 0724-8741 14:10<1479 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer