caa a22 4500 477127142 CHVBK 20180405111653.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1012100100865 doi (NATIONALLICENCE)springer-10.1023/A:1012100100865 Iontophoresis of Poly-L-lysines: The Role of Molecular Weight? [Elektronische Daten] [Norris Turner, Laura Ferry, Matthew Price, Christopher Cullander, Richard Guy] Purpose. (1) To determine the extent of iontophoretic transport as a function of molecular weight (MW) of the penetrant; and (2) to visually and quantitatively characterize the iontophoretic transport pathways (follicular (F) versus nonfollicular (NF)) of the fluorescently-labeled poly-L-lysines employed. Methods. A series of fluorescently-labeled poly-L-lysines (FITC-PLLs) [4 KDa, 7 KDa and 26 KDa] were used to study the extent and distribution of iontophoretic skin penetration as a function of MW using laser scanning confocal microscopy (LSCM). Results. It was found that, relative to the passive controls, and under the electrical conditions considered, iontophoresis greatly enhanced the penetration of the 4 KDa analog, slightly elevated the delivery of the 7 KDa FITC-PLL, but had no effect on the transport of the larger 26 KDa FITC-PLL. Quantitative analyses of LSCM images revealed that iontophoresis increased transport via F pathways only slightly more than that through NF pathways for the 4 KDa and 7 KDa FITC-PLL molecules. Conclusions. It is visually apparent that the iontophoretic transport pathways taken are importantly determined by the physicochemical properties (including size and charge) of the penetrant. The results presented here demonstrate an inverse dependence of iontophoretic delivery upon the MW of the penetrant. Plenum Publishing Corporation, 1997 iontophoresis nationallicence molecular weight nationallicence skin nationallicence confocal microscopy nationallicence drug delivery nationallicence Turner Norris Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut Ferry Laura Drug Delivery Systems, Novartis, 07936, East Hanover, New Jersey aut Price Matthew Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut Cullander Christopher Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut Guy Richard Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/10(1997-10-01), 1322-1331 0724-8741 14:10<1322 1997 14 11095 https://doi.org/10.1023/A:1012100100865 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012100100865 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turner Norris Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 700 1- Ferry Laura Drug Delivery Systems, Novartis, 07936, East Hanover, New Jersey aut NATIONALLICENCE 700 1- Price Matthew Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 700 1- Cullander Christopher Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 700 1- Guy Richard Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California-San Francisco, 94143-0446, San Francisco, California aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/10(1997-10-01), 1322-1331 0724-8741 14:10<1322 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 222385162 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer