caa a22 4500 477127363 CHVBK 20180405111653.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1012151616910 doi (NATIONALLICENCE)springer-10.1023/A:1012151616910 Non-Polarized Secretion of Mouse Interferon-β from Gene-Transferred Human Intestinal Caco-2 Cells [Elektronische Daten] [Kenji Kawabata, Mayumi Kondo, Yoshihiko Watanabe, Yoshinobu Takakura, Mitsuru Hashida] Purpose. The intestinal epithelium is considered to be a feasible target for somatic gene therapy. To this end, Caco-2 cells derived from human colon carcinoma were transfected with a mouse interferon-β (IFN-β) expression vector and several stable sublines were established; this hetero-specific cytokine allows unexpected cellular effects to be avoided. Using the highest mouse IFN-β-producing sublines, the mode of IFN secretion was examined. Methods. The secretion polarity of mouse IFN-β in its gene-transduced Caco-2 sublines was studied in a bicameral culture system in which the chambers were separated by microporous filters. Results. Mouse IFN-β was secreted to the same extent from both apical and basolateral surfaces of the transduced cells regardless of cell aging. Conclusions. These results suggest that in the intestinal epithelium exogenous gene products such as IFNs can be delivered to both the luminal and blood sides in vivo. Thus, the intestinal epithelium may be suitable for systemic and local delivery of therapeutic proteins by gene transfer. Plenum Publishing Corporation, 1997 gene transfer nationallicence interferon-β nationallicence Caco-2 cells nationallicence non-polarized secretion nationallicence gene therapy nationallicence Kawabata Kenji Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut Kondo Mayumi Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut Watanabe Yoshihiko Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut Takakura Yoshinobu Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut Hashida Mitsuru Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/4(1997-04-01), 483-485 0724-8741 14:4<483 1997 14 11095 https://doi.org/10.1023/A:1012151616910 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012151616910 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawabata Kenji Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Kondo Mayumi Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Watanabe Yoshihiko Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Takakura Yoshinobu Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Hashida Mitsuru Department of Drug Delivery Research, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/4(1997-04-01), 483-485 0724-8741 14:4<483 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer