caa a22 4500 477127517 CHVBK 20180405111654.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1012095431931 doi (NATIONALLICENCE)springer-10.1023/A:1012095431931 Splenic Trapping of Nanoparticles: Complementary Approaches for In Situ Studies [Elektronische Daten] [Marina Demoy, Stéphane Gibaud, Jean Andreux, Colette Weingarten, Bruno Gouritin, Patrick Couvreur] Purpose. To identify more accurately in the spleen, the areas and the cells where nanoparticulate carriers were taken up from the blood flow, a series of complementary approaches were used. Methods. First,in and ex vivo examination of the whole spleen led to a global view of all the trapping areas. Then, histological studies on frozen sections of the same organ allowed for a more precise localization of these areas and image analysis gave an evaluation of tissue distribution of the nanoparticles. Finally, immunological and enzymological characteristics of the capturing cells were determined in situ, using monoclonal antibodies (F4/80 and anti-sialoadhesin) and cyto-chemichal reactions (esterases and acid phosphatase). Furthermore incubation of spleen slices with different nanoparticles was used so as to know if the capture was due to a high capturing capacity of these cells or to a high blood flow in their vicinity. Results. It was shown that more than 90% of the splenic capture was localized in the marginal zone of the follicles. The capturing cells form a special population of macrophages inserted in a reticular meshwork, showing low esterase and acid phosphatase activities, giving faint or no reaction with F4/80 or anti-sialoadhesin antibodies. The circulating nanoparticles were quickly trapped with rather low specificity by these cells. Conclusions. Combination of coherent approaches allowed for the tracking of capturing cells from in vivo observations to their in situ identification on immunological and enzymological criteria. Plenum Publishing Corporation, 1997 spleen nationallicence nanoparticles nationallicence capturing cells nationallicence histoenzymology nationallicence immunohistochemistry nationallicence mouse nationallicence Demoy Marina Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut Gibaud Stéphane Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut Andreux Jean Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut Weingarten Colette Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut Gouritin Bruno Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut Couvreur Patrick Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/4(1997-04-01), 463-468 0724-8741 14:4<463 1997 14 11095 https://doi.org/10.1023/A:1012095431931 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012095431931 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Demoy Marina Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut NATIONALLICENCE 700 1- Gibaud Stéphane Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut NATIONALLICENCE 700 1- Andreux Jean Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut NATIONALLICENCE 700 1- Weingarten Colette Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut NATIONALLICENCE 700 1- Gouritin Bruno Laboratoire d'Hématologie, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296, Châtenay-Malabry cedex, France aut NATIONALLICENCE 700 1- Couvreur Patrick Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, URA CNRS 1218, Faculté de Pharmacie, Université Paris XI, 5, Rue J.B. Clément, 92 296 Châtenay, Malabry cedex, France aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/4(1997-04-01), 463-468 0724-8741 14:4<463 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer