caa a22 4500 477127878 CHVBK 20180405111655.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1012117531448 doi (NATIONALLICENCE)springer-10.1023/A:1012117531448 Preparation of Biodegradable, Surface Engineered PLGA Nanospheres with Enhanced Lymphatic Drainage and Lymph Node Uptake [Elektronische Daten] [Ann Hawley, Lisbeth Illum, Stanley Davis] Purpose. Nanospheres can be utilised for the targeting of drugs and diagnostic agents to the regional lymph nodes. The surface modification of model polystyrene, (PS), and poly(lactide-co-glycolide),(PLGA), nanospheres by poly(lactide)-poly(ethylene glycol), (PLA:PEG), copolymers has been assessed by in vitro characterisation and in vivobiodistribution studies following subcutaneous administration of the nanospheres to the rat. Methods. Three PLA: PEG copolymers were investigated, with PEG chain lengths of 750, 2000 and 5000 Da. The PLA:PEG copolymers were either coated onto the surface of PS and PLGA nanospheres or used as a co-precipitate in the formation of PLGA-PLA:PEG nanospheres. Coating of the nanospheres was confirmed by an increase in their particle size and a corresponding decrease in the surface potential. The kinetics of injection site drainage and lymph node retention was determined over a 24 hour time course for naked, coated and co-precipitated nanosphere systems. Results. Dependent on the surface characteristics, the distribution of the nanospheres can be significantly modified and the lymph node localisation dramatically enhanced by coating their surfaces with PLA:PEG copolymers or by producing co-precipitate nanospheres of PLGA and PLA:PEG. Conclusions. A fully biodegradable nanosphere system has been developed with excellent lymph node targeting characteristics. Plenum Publishing Corporation, 1997 lymph node targeting nationallicence subcutaneous administration nationallicence poly(lactide-co-glycolide) nationallicence poly(lactide)-poly(ethylene glycol) copolymers nationallicence polystyrene nationallicence nanospheres nationallicence Hawley Ann Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut Illum Lisbeth Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut Davis Stanley Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/5(1997-05-01), 657-661 0724-8741 14:5<657 1997 14 11095 https://doi.org/10.1023/A:1012117531448 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012117531448 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hawley Ann Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut NATIONALLICENCE 700 1- Illum Lisbeth Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut NATIONALLICENCE 700 1- Davis Stanley Department of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/5(1997-05-01), 657-661 0724-8741 14:5<657 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer