caa a22 4500 477127924 CHVBK 20180405111655.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1012153011884 doi (NATIONALLICENCE)springer-10.1023/A:1012153011884 Enhanced Oral Uptake of Tomato Lectin-Conjugated Nanoparticles in the Rat [Elektronische Daten] [Nasir Hussain, Praful Jani, Alexander Florence] Purpose. To investigate the usefulness of a surface-conjugated, bioadhesive molecule, tomato lectin, to augment intestinal uptake of orally administered inert nanoparticles. Methods. Fluorescent 500 nm polystyrene nanoparticles with tomato lectin covalently surface coupled using a carbodiimide reaction were administered to female Wistar rats by oral gavage daily for 5 days. Results. Analysis of tissue extracted polymer by gel permeation chromatography revealed a 23% systemic uptake of tomato lectin conjugated nanoparticles compared to < 0.5% of TL nanoparticles blocked with N-acetylchitotetraose thus representing an increase of almost 50 fold across the intestine. Intestinal uptake of tomato lectin-conjugated nanoparticles via the villous tissue was 15 times higher than uptake by the gut-associated lymphoid tissue. Conclusions. The application of tomato lectin as a bioadhesive agent in vivo has been demonstrated to enhance subsequent intestinal transcytosis of colloidal particulates to which it is bound. Plenum Publishing Corporation, 1997 tomato lectin nationallicence Peyer's patches nationallicence nanoparticles nationallicence oral uptake nationallicence bioadhesion nationallicence Hussain Nasir Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut Jani Praful Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut Florence Alexander Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/5(1997-05-01), 613-618 0724-8741 14:5<613 1997 14 11095 https://doi.org/10.1023/A:1012153011884 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012153011884 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hussain Nasir Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut NATIONALLICENCE 700 1- Jani Praful Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut NATIONALLICENCE 700 1- Florence Alexander Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, United Kingdom aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/5(1997-05-01), 613-618 0724-8741 14:5<613 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer