caa a22 4500 477128203 CHVBK 20180405111656.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1012004911942 doi (NATIONALLICENCE)springer-10.1023/A:1012004911942 Characterization of Pulmonary Alveolar Esterases of the Primate Cercopithecus pygerythrus [Elektronische Daten] [Henry Leng, James Syce] Purpose. To evaluate and classify the hydrolases of the primate lung. Methods. Homologous series of aromatic, aliphatic, and choline ester substrates were assayed with the pH-stat method to obtain the Michaelis-Menten kinetic constants, Vmax and Km, for the enzymes in pulmonary alveolar tissue with esterase activity. Polyacrylamide gel electrophoresis was employed to determine the number of such hydrolytic enzymes. Inhibition studies with selective esterase inhibitors were carried out to classify enzymes as either arylesterases, carboxylesterases, or cholinesterases. Results. Aromatic, aliphatic, and choline ester drugs were hydrolyzed by alveolar tissue of the primate lung. The catalytic enzymes were more specific for aromatic esters since these were metabolized at faster rates than the other substrates. Aromatic ester hydrolysis was also inhibited by triorthocresylphosphate (TOCP), a potent inhibitor of carboxylesterases. Inhibitors of arylesterases and cholinesterases had minimal effect on the enzymic hydrolysis of all substrates. Polyacrylamide gel electrophoresis demonstrated three enzymes to have esterolytic activity, two (MWs 269 and 281 kDa) of which were sensitive to TOCP and are therefore carboxylesterases. The third enzyme (MW 34 kDa), was unaffected by esterase inhibitors and, thus, cannot be classified as an esterase. Conclusions. Primate pulmonary alveolar tissue contains two isozymes of carboxylesterases. Plenum Publishing Corporation, 1997 carboxylesterases nationallicence ester prodrugs nationallicence pulmonary esterases nationallicence primate nationallicence vervet monkey nationallicence Leng Henry aut Syce James aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/2(1997-02-01), 203-207 0724-8741 14:2<203 1997 14 11095 https://doi.org/10.1023/A:1012004911942 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012004911942 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leng Henry aut NATIONALLICENCE 700 1- Syce James aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/2(1997-02-01), 203-207 0724-8741 14:2<203 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer