caa a22 4500 477128394 CHVBK 20180405111656.0 cr unu---uuuuu 170330e19971101xx s 000 0 eng 10.1023/A:1012161814494 doi (NATIONALLICENCE)springer-10.1023/A:1012161814494 Stereoselectivity of Antibodies for the Bioanalysis of Chiral Drugs [Elektronische Daten] [Pierre Got, Jean-Michel Scherrmann] The use of immunoassay techniques represents an alternative approach to the more common chromatographic methods for the determination of the concentrations of chiral drugs. In the development of the former technique, the inherent stereoselectivity of the antibodies used appears to be an important parameter to be studied. The structural features of the drug, including the environment around the asymmetric center, the flexibility of the molecule and the ability of the molecule to undergo racemization, contribute to the molecule's ability to be recognized by stereoselective antibodies. These parameters are intrinsic and can not be influenced by the investigator. On the other hand, other parameters can be modified to favor the raising of highly stereoselective antibodies. These include the synthesis of an appropriate hapten; the selection of an optimal spacer arm between the hapten and the carrier protein used for the immunization procedure; and the choice of appropriate immunization and antibody-screening procedures. The purification of antibodies using affinity chromatography may also facilitate the selection of stereoselective antibodies. Plenum Publishing Corporation, 1997 monoclonal antibodies nationallicence polyclonal antibodies nationallicence stereoselectivity nationallicence chiral drugs nationallicence immunoassay nationallicence chiral analysis nationallicence Got Pierre Département de Bio-Analyse, Technologic Servier, 25-27 rue Eugène Vignat, F-45000, Orléans, France aut Scherrmann Jean-Michel INSERM, Unité 26, 200 rue du Faubourg St Denis, F-75010 Paris, France and Faculté de Pharmacie, 4 Avenue de 1'Observatoire, F-75006, Paris, France aut Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/11(1997-11-01), 1516-1523 0724-8741 14:11<1516 1997 14 11095 https://doi.org/10.1023/A:1012161814494 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1012161814494 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Got Pierre Département de Bio-Analyse, Technologic Servier, 25-27 rue Eugène Vignat, F-45000, Orléans, France aut NATIONALLICENCE 700 1- Scherrmann Jean-Michel INSERM, Unité 26, 200 rue du Faubourg St Denis, F-75010 Paris, France and Faculté de Pharmacie, 4 Avenue de 1'Observatoire, F-75006, Paris, France aut NATIONALLICENCE 773 0- Pharmaceutical Research Kluwer Academic Publishers-Plenum Publishers 14/11(1997-11-01), 1516-1523 0724-8741 14:11<1516 1997 14 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer