caa a22 4500 477132308 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1018362603065 doi (NATIONALLICENCE)springer-10.1023/A:1018362603065 Zinc protection against cadmium-induced infertility in female rats. Effect of zinc and cadmium on the progesterone production of cultured granulosa cells [Elektronische Daten] [Katalin Paksy, Bertalan Varga, Péter Lazar] Adult female rats were treated subcutaneously (sc) with zinc chloride (ZnCl, 10 or 20 mg kg body weight, bw) four times during two ovarian cycles. The third injection was accompanied by cadmium chloride (CdCl) administration sc (2.5, 5 and 10 mg kg bw). The fourth zinc (Zn) treatment was followed by mating. ZnCl (20 mg kg) itself impaired fertility by 20%, while CdCl dose-dependently blocked the receptivity of female rats. In combination with 2.5 and 5 mg kg CdCl the metal salts decreased fertility in an additive fashion, whereas at the highest CdCl dose (10 mg kg) a marked ameliorating effect of ZnCl (10 and 20 mg kg) on cadmium (Cd)-caused sterility was observed. In the pregnant animals apart from the higher Cd-induced blood progesterone levels and reduced body weight gain of dams, no significant treatment-related maternal and fetal effects could be observed. ZnCl (10 to 80 μm) and CdCl (10 to 80 μm) were added to the culture medium of ovarian granulosa cells. CdCl suppressed follicle-stimulating-hormone- (FSH-) and cAMP-stimulated progesterone accumulation. No protective effect of Zn against Cd-induced drop in progesterone production could be seen, while Zn by itself induced a significant increase in FSH-supported progesterone synthesis. In conclusion, while Zn protected against Cd-induced sterility in vivo, it failed to counteract the direct effect of Cd on steroid biosynthesis. The data indicate that Zn protection does not take place at the level of ovary. Moreover, Zn and Cd seem to affect FSH-stimulated progesterone production by different mechanisms. Chapman and Hall, 1997 cadmium nationallicence progesterone nationallicence sterility nationallicence zinc-FSH interaction nationallicence zinc protection nationallicence Paksy Katalin National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut Varga Bertalan National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut Lazar Péter National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut Biometals Kluwer Academic Publishers 10/1(1997-03-01), 27-36 0966-0844 10:1<27 1997 10 10534 https://doi.org/10.1023/A:1018362603065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018362603065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paksy Katalin National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut NATIONALLICENCE 700 1- Varga Bertalan National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut NATIONALLICENCE 700 1- Lazar Péter National Institute of Occupational Health, Budapest, Hungary (+36) 1 215 7890 aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/1(1997-03-01), 27-36 0966-0844 10:1<27 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer