caa a22 4500 477132316 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1018306502157 doi (NATIONALLICENCE)springer-10.1023/A:1018306502157 Effects of selenium and serum on selenoprotein W in cultured L8 muscle cells [Elektronische Daten] [Jan-Ying Yeh, Bor-Rung Ou, Neil Forsberg, Philip Whanger] When rat L8 muscle cells were cultured to examine the effects of serum and selenium concentration on selenoprotein W levels and glutathione peroxidase (GPX) activities, no significant differences (P > 0.05) were found in selenoprotein W levels and GPX activities during differentiation. With three different forms of selenium, selenoprotein W levels and GPX activities were shown to increase in L8 myotubes cultured in media with these selenocompounds. Selenite was utilized more efficiently than selenocysteine for both selenoprotein W and GPX activity, but selenium as selenomethionine was less available. Both the protein content and mRNA levels for selenoprotein W were affected by the selenium content of the media. Northern blot data indicated that the expression of selenoprotein W mRNA increased significantly when L8 myotubes were cultured with selenium (P > 0.05). L8 myotubes cultured in 10% calf serum (CS) versus 2% CS with or without addition of 10 m selenium indicated that the increase of selenoprotein W level in L8 myotubes cultured with higher serum concentration (10% CS) is due to the higher selenium concentration in media rather than serum itself. Chapman and Hall, 1997 glutathione peroxidase nationallicence myotubes nationallicence Northern blots nationallicence selenite nationallicence selenocysteine nationallicence selenomethionine nationallicence selenoprotein W nationallicence Western blots nationallicence Yeh Jan-Ying Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut Ou Bor-Rung Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut Forsberg Neil Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut Whanger Philip Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut Biometals Kluwer Academic Publishers 10/1(1997-03-01), 11-22 0966-0844 10:1<11 1997 10 10534 https://doi.org/10.1023/A:1018306502157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018306502157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeh Jan-Ying Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Ou Bor-Rung Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Forsberg Neil Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Whanger Philip Departments of Agricultural Chemistry and Animal Sciences, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/1(1997-03-01), 11-22 0966-0844 10:1<11 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer